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BCH 6744 |
Molecular Structure Determination by X-Ray Crystallography
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Fall Semester 2007 |
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Lectures ("L" hereafter): Held Mondays (M), Wednesdays (W), and Fridays (F), 4th period (10:40 am to 11:30 am)in LG-110A (McKnight Brain Institute). |
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Coordinator: |
Mavis Agbandje-McKenna |
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LG-179 Brain Ins |
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392-5694 |
| E-mail:
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mckenna@ufl.edu |
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Introduction:
Course Description The objective of the course is to provide detailed theoretical and practical instructions on the methodology of X-ray crystallography, a biophysical technique at the forefront of research efforts aimed at the structure-function elucidation of macromolecules. Students will learn the theory behind the technique of X-ray crystallography and will apply the knowledge obtained to the three-dimensional structure determination of a macromolecule (lysozyme). The laboratory class will provide practical experience in sample preparation, operation of the instrumentation, data acquisition, data analysis, phasing and refinement.
The anticipation is that students will take both the theoretical and practical sections of the course for 2 credits, which will run concurrently. However, students may earn 1 credit by either attending the lectures on the theory of X-ray crystallography or the laboratory class. This hands-on approach will reinforce the applicability of this methodology in the analysis of the functional properties of a biological macromolecule.
Meeting Times and Places: Lectures (L): Held Mondays (M), Wednesdays (W) and Fridays (F), 4th period (10:40 am to 11:30 am) in LG-110A (M) and LG-116 (W & F). Laboratory practicals (P): Held Thursdays, 4th to 6th period (10:40 am to 1:30 pm) in LG-171.
Prerequisites : BCH 6740 or equivalent, or consent of instructor.
Recommended text: Rhodes G. "Crystallography Made Crystal Clear". Academic Press, Inc.
Blow, D. "Outline of Crystallography for Biologist". Oxford University Press.
Tests and Grading: Lecture component will be graded based on take home problems and a final take home exam. Laboratory component grade will be 30% homework and 70% completed lab. Project report written in the form for crystal structure manuscript (Acta Cryst. D format).
Course Notes: http://www.mbi.ufl.edu/facilities/msg/courses.html
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COURSE
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COURSE
EVALUATION |
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SCHEDULE |
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EXAM
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DATE
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TIME
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LECTURE
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ROOM
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Oct. 06 |
2:00 pm |
All lectures |
LG-179 |
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CORE INSTRUCTORS: |
Dr. Robert McKenna (RM)
Office: LG-179, phone 392-5696
e-mail: rmckenna@ufl.edu
Dr. Mavis Agbanndje-McKenna (MAM)
Office: LG-179, 392-5694
e-mail: mckenna@ufl.edu
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