Mingyi Xie, Ph.D.

Assistant Professor

 Mingyi Xi picture

Research: Gene expression regulation by non-coding RNAs; microRNA biogenesis

Ph.D from Arizona State University, 2010


Contact Information:

Office:  263 CGRC
Lab:  265 CGRC
Telephone: 273-8171
Email:  mingyi.xie@ufl.edu

 

Dr. Xie’s Lab

BIOGRAPHY

From 2005 to 2010, Dr. Xie’s Ph.D. work focused on investigating the structure and function of vertebrate telomerase, under the guidance of Dr. Julian Chen at Arizona State University. In 2010, he joined the laboratory of Dr. Joan Steitz at Yale University to study non-canonical microRNA (miRNA) biogenesis in Herpesviruses and their mammalian hosts. Dr. Xie joined the faculty at the University of Florida in September, 2016.

RESEARCH DESCRIPTION

MiRNAs are ~22 nucleotide (nt) ubiquitous gene regulators that modulate diverse cellular pathways including differentiation, proliferation and apoptosis, all critical to human development and diseases. Canonical miRNAs are produced from long primary (pri-) miRNA transcripts that are cleaved by the nuclear Microprocessor complex, with the resulting precursor (pre-) miRNA hairpins exported by Exportin-5 and processed by cytoplasmic Dicer to yield mature miRNAs (Fig. 1, middle panel). At Yale, Dr. Xie documented two surprising miRNA biogenesis pathways:

  • Herpesvirus saimiri (HVS)-miRNAs are processed by the host Integrator complex, a 14-subunit complex best known for executing the 3′-end cleavage of cellular small nuclear RNAs (snRNAs), therefore bypassing the canonical Microprocessor cleavage. (Fig. 1, top panel)
  • Mammalian m7G-capped precursor miRNAs are generated from RNA polymerase (Pol) II transcription initiation sites. Whereas the nuclear-cytoplasmic export of capped pre-miRNAs is mediated by Exportin-1. (Fig.1 bottom panel)

These pathways surprisingly incorporate fundamental cellular machineries involved in processing other classes of RNAs, expanding our appreciation of their impact on small RNA populations and oncogenesis. Our group’s immediate research goal is to further delineate the unique modes of miRNA production, including Integrator-mediated RNA metabolism, and understand the functions of these special miRNAs in herpesviruses and their hosts. Such research will allow the design of therapies for related oncogenic herpesviruses and cancers.

Figure 1. The canonical miRNA biogenesis pathway (middle panel) is compared to alternative pathways for HVS miRNAs (top panel) and m7G-capped pre-miRNAs (bottom panel). Mature miRNAs are in orange.