Antonette D Bennett

Antonette D Bennett,

AST SCTST

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 846-2001
Business Email: dendena@ufl.edu

Teaching Profile

Courses Taught
2021-2023
BCH6936 Biochemistry Seminar
2021
GMS6001 Fundamentals of Biomedical Sciences I
2022-2023
BCH4024 Introduction to Biochemistry and Molecular Biology

Publications

2021
Comparative structural, biophysical, and receptor binding study of true type and wild type AAV2.
Journal of structural biology. 213(4) [DOI] 10.1016/j.jsb.2021.107795. [PMID] 34509611.
2021
Effects of Altering HSPG Binding and Capsid Hydrophilicity on Retinal Transduction by AAV.
Journal of virology. 95(10) [DOI] 10.1128/JVI.02440-20. [PMID] 33658343.
2020
Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking.
Viruses. 12(6) [DOI] 10.3390/v12060668. [PMID] 32575696.
2020
Attenuation of Heparan Sulfate Proteoglycan Binding Enhances In Vivo Transduction of Human Primary Hepatocytes with AAV2.
Molecular therapy. Methods & clinical development. 17:1139-1154 [DOI] 10.1016/j.omtm.2020.05.004. [PMID] 32490035.
2020
Geminivirus structure and assembly.
Advances in virus research. 108:1-32 [DOI] 10.1016/bs.aivir.2020.09.005. [PMID] 33837714.
2020
Restoring the natural tropism of AAV2 vectors for human liver.
Science translational medicine. 12(560) [DOI] 10.1126/scitranslmed.aba3312. [PMID] 32908003.
2020
Structure comparison of the chimeric AAV2.7m8 vector with parental AAV2.
Journal of structural biology. 209(2) [DOI] 10.1016/j.jsb.2019.107433. [PMID] 31859208.
2019
Adeno-Associated Virus VP1u Exhibits Protease Activity.
Viruses. 11(5) [DOI] 10.3390/v11050399. [PMID] 31035643.
2019
Protease-Activatable Adeno-Associated Virus Vector for Gene Delivery to Damaged Heart Tissue.
Molecular therapy : the journal of the American Society of Gene Therapy. 27(3):611-622 [DOI] 10.1016/j.ymthe.2019.01.015. [PMID] 30772143.
2018
A novel adeno-associated virus capsid with enhanced neurotropism corrects a lysosomal transmembrane enzyme deficiency.
Brain : a journal of neurology. 141(7):2014-2031 [DOI] 10.1093/brain/awy126. [PMID] 29788236.
2018
AAV6 K531 serves a dual function in selective receptor and antibody ADK6 recognition.
Virology. 518:369-376 [DOI] 10.1016/j.virol.2018.03.007. [PMID] 29604478.
2018
Assembly and disassembly intermediates of maize streak geminivirus.
Virology. 525:224-236 [DOI] 10.1016/j.virol.2018.09.011. [PMID] 30300759.
2018
Atomic structure of a rationally engineered gene delivery vector, AAV2.5.
Journal of structural biology. 203(3):236-241 [DOI] 10.1016/j.jsb.2018.05.004. [PMID] 29775653.
2017
Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS.
Molecular therapy. Nucleic acids. 8:184-197 [DOI] 10.1016/j.omtn.2017.06.011. [PMID] 28918020.
2017
Thermal Stability as a Determinant of AAV Serotype Identity.
Molecular therapy. Methods & clinical development. 6:171-182 [DOI] 10.1016/j.omtm.2017.07.003. [PMID] 28828392.
2016
Cryo-electron Microscopy Reconstruction and Stability Studies of the Wild Type and the R432A Variant of Adeno-associated Virus Type 2 Reveal that Capsid Structural Stability Is a Major Factor in Genome Packaging.
Journal of virology. 90(19):8542-51 [DOI] 10.1128/JVI.00575-16. [PMID] 27440903.
2016
Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors.
Journal of virology. 90(8):4215-4231 [DOI] 10.1128/JVI.00200-16. [PMID] 26865709.
2016
Microbatch Mixing: “Shaken Not Stirred”, a Method for Macromolecular Microcrystal Production for Serial Crystallography
Crystal Growth & Design. 16(11):6214-6221 [DOI] 10.1021/acs.cgd.6b00643.
2016
Mutants at the 2-Fold Interface of Adeno-associated Virus Type 2 (AAV2) Structural Proteins Suggest a Role in Viral Transcription for AAV Capsids.
Journal of virology. 90(16):7196-7204 [DOI] 10.1128/JVI.00493-16. [PMID] 27252527.
2014
Defining the stoichiometry and cargo load of viral and bacterial nanoparticles by Orbitrap mass spectrometry.
Journal of the American Chemical Society. 136(20):7295-9 [DOI] 10.1021/ja502616y. [PMID] 24787140.
2014
OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.
Human gene therapy. 25(3):212-22 [DOI] 10.1089/hum.2013.184. [PMID] 24299301.
2013
Capsid antibodies to different adeno-associated virus serotypes bind common regions.
Journal of virology. 87(16):9111-24 [DOI] 10.1128/JVI.00622-13. [PMID] 23760240.
2013
Comparative analysis of adeno-associated virus capsid stability and dynamics.
Journal of virology. 87(24):13150-60 [DOI] 10.1128/JVI.01415-13. [PMID] 24067976.
2013
Construction, expression, and purification of recombinant αVβ5 integrin.
Protein expression and purification. 89(2):225-31 [DOI] 10.1016/j.pep.2013.04.002. [PMID] 23583935.
2013
Molecular cloning, overexpression, and an efficient one-step purification of α5β1 integrin.
Protein expression and purification. 92(1):21-8 [DOI] 10.1016/j.pep.2013.08.013. [PMID] 23993979.
2013
Structure and dynamics of adeno-associated virus serotype 1 VP1-unique N-terminal domain and its role in capsid trafficking.
Journal of virology. 87(9):4974-84 [DOI] 10.1128/JVI.02524-12. [PMID] 23427155.
2012
Evidence for pH-dependent protease activity in the adeno-associated virus capsid.
Journal of virology. 86(21):11877-85 [DOI] 10.1128/JVI.01717-12. [PMID] 22915820.
2012
Structural insight into the unique properties of adeno-associated virus serotype 9.
Journal of virology. 86(12):6947-58 [DOI] 10.1128/JVI.07232-11. [PMID] 22496238.
2012
The 3D Structure of Adeno-Associated Virus Serotype 9 and Its Unique Properties
Molecular Therapy. 20
2011
Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina.
Molecular therapy : the journal of the American Society of Gene Therapy. 19(2):293-301 [DOI] 10.1038/mt.2010.234. [PMID] 21045809.
2009
Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9.
Acta crystallographica. Section F, Structural biology and crystallization communications. 65(Pt 7):715-8 [DOI] 10.1107/S1744309109021460. [PMID] 19574648.
2009
Towards Characterizing Vector-Host Interactions: Adeno-Associated Virus Capsids in Complex With Antibody Fragments
Molecular Therapy. 17
2008
A Comparative Analysis of the Structural Architecture of Ssdna Viruses
Computational and Mathematical Methods in Medicine. 9(34):183-196 [DOI] 10.1080/17486700802168247.

Grants

Dec 2021 – Jan 2022
Generation of AAV1 VP3-only DNA empty capsids
Role: Co-Investigator
Funding: THERMO FINNIGAN
Aug 2021 – Oct 2021
Generation of AAV1 VP3 only DNA empty capsids
Role: Co-Investigator
Funding: THERMO FINNIGAN
Jun 2020 – May 2022
Adverum Task Order #2 – Structure determination of AAV variants and 3D homology model building
Role: Co-Investigator
Funding: ADVERUM BIOTECHNOLOGIES INC
Apr 2020 ACTIVE
Correlative structure and function studies of adeno-associated virus particles during viral genome release
Role: Co-Investigator
Funding: NATL INST OF HLTH NIAID

Contact Details

Phones:
Business:
(352) 846-2001
Emails:
Business:
dendena@ufl.edu
Addresses:
Business Mailing:
1600 SW ARCHER RD
GAINESVILLE FL 32610
Business Street:
BRAIN INSTITUTE RM#LG171
ANTONETTE BENNETT
GAINESVILLE FL 32603