Melike Caglayan

Melike Caglayan,

Assistant Professor

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 294-8383
Business Email: caglayanm@ufl.edu

Accomplishments

Annual Meeting Early Career Travel Award
2022 · ASBMB (The American Society for Biochemistry and Molecular Biology)
Early Career Faculty Award
2021 · American Society for Biochemistry and Molecular Biology (ASBMB)
Thomas Maren Junior Investigator Award
2020 · University of Florida, College of Medicine
NIH Intramural Research Program Paper
2017 · NIH Catalyst
Pathway to Independence Award (K99/R00)
2015 · National Institute of Health (NIH)
Intramural Paper of the Month, NIEHS
2014 · The National Institute of Environmental Health Sciences
Doctoral Dissertation Award
2010 · The Scientific and Technological Research Council of Turkey
PhD visiting scholarship
2009 · Yale Medical School

Teaching Profile

Courses Taught
2020-2021,2023
BCH4905 Biochemistry Senior Research
2020-2023
BCH5413 Mammalian Molecular Biology and Genetics
2020-2021
HSC4970 Public Health and Health Professions Senior Honors Thesis
2018
MCB7979 Advanced Research
2021-2022
BCH6415 Advanced Molecular and Cell Biology
2022-2023
GMS6001 Fundamentals of Biomedical Sciences I

Publications

2022
Structures of LIG1 that engage with mutagenic mismatches inserted by polβ in base excision repair.
Nature communications. 13(1) [DOI] 10.1038/s41467-022-31585-w. [PMID] 35790757.
2021
DNA ligase I fidelity mediates the mutagenic ligation of pol β oxidized and mismatch nucleotide insertion products in base excision repair.
The Journal of biological chemistry. 296 [DOI] 10.1016/j.jbc.2021.100427. [PMID] 33600799.
2021
The scaffold protein XRCC1 stabilizes the formation of polβ/gap DNA and ligase IIIα/nick DNA complexes in base excision repair.
The Journal of biological chemistry. 297(3) [DOI] 10.1016/j.jbc.2021.101025. [PMID] 34339737.
2020
DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage.
Mutagenesis. [DOI] 10.1093/mutage/geaa023. [PMID] 32914844.
2020
Pol β gap filling, DNA ligation and substrate-product channeling during base excision repair opposite oxidized 5-methylcytosine modifications.
DNA repair. 95 [DOI] 10.1016/j.dnarep.2020.102945. [PMID] 32853828.
2020
Pol μ ribonucleotide insertion opposite 8-oxodG facilitates the ligation of premutagenic DNA repair intermediate.
Scientific reports. 10(1) [DOI] 10.1038/s41598-020-57886-y. [PMID] 31969622.
2020
The ligation of pol β mismatch insertion products governs the formation of promutagenic base excision DNA repair intermediates.
Nucleic acids research. 48(7):3708-3721 [DOI] 10.1093/nar/gkaa151. [PMID] 32140717.
2019
Interplay between DNA Polymerases and DNA Ligases: Influence on Substrate Channeling and the Fidelity of DNA Ligation.
Journal of molecular biology. 431(11):2068-2081 [DOI] 10.1016/j.jmb.2019.04.028. [PMID] 31034893.
2018
Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate.
Nature communications. 9(1) [DOI] 10.1038/s41467-018-06700-5. [PMID] 30310068.
2018
XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity.
DNA repair. 64:26-33 [DOI] 10.1016/j.dnarep.2018.02.004. [PMID] 29477978.
2017
Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts.
Nucleic acids research. 45(17):10079-10088 [DOI] 10.1093/nar/gkx654. [PMID] 28973450.
2017
DNA polymerase β: A missing link of the base excision repair machinery in mammalian mitochondria.
DNA repair. 60:77-88 [DOI] 10.1016/j.dnarep.2017.10.011. [PMID] 29100041.
2017
Oxidized nucleotide insertion by pol β confounds ligation during base excision repair.
Nature communications. 8 [DOI] 10.1038/ncomms14045. [PMID] 28067232.
2017
Role of DNA polymerase β oxidized nucleotide insertion in DNA ligation failure.
Journal of radiation research. 58(5):603-607 [DOI] 10.1093/jrr/rrx027. [PMID] 28992331.
2016
Impact of Ribonucleotide Backbone on Translesion Synthesis and Repair of 7,8-Dihydro-8-oxoguanine.
The Journal of biological chemistry. 291(46):24314-24323 [PMID] 27660390.
2015
Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair.
DNA repair. 35:85-9 [DOI] 10.1016/j.dnarep.2015.09.010. [PMID] 26466358.
2014
Base excision repair of tandem modifications in a methylated CpG dinucleotide.
The Journal of biological chemistry. 289(20):13996-4008 [DOI] 10.1074/jbc.M114.557769. [PMID] 24695738.
2014
Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair.
Nature structural & molecular biology. 21(5):497-9 [DOI] 10.1038/nsmb.2818. [PMID] 24777061.

Grants

Aug 2022 ACTIVE
DNA ligase activities during base excision repair coordination
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS
Apr 2020 – Dec 2020
Structural and Mechanistic Studies of Base Excision DNA Repair
Role: Principal Investigator
Funding: UF FOUNDATION
Aug 2018 – Jul 2022
Oxidant and environmental toxicant-induced effects compromise ligation in DNA repair
Role: Principal Investigator
Funding: NATL INST OF HLTH NIEHS

Contact Details

Phones:
Business:
(352) 294-8383
Emails:
Business:
caglayanm@ufl.edu
Addresses:
Business Mailing:
1200 NEWELL DRIVE ARB R3-226A
GAINESVILLE FL 326100001