Melike Caglayan,
Assistant Professor
Department:
MD-BIOCHEM / MOLECULAR BIOL
Business Phone:
(352) 294-8383
Business Email:
caglayanm@ufl.edu
Accomplishments
Thomas Maren Junior Investigator Award
2020
·
University of Florida, College of Medicine
NIH Intramural Research Program Paper
2017
·
NIH Catalyst
Pathway to Independence Award (K99/R00)
2015
·
National Institute of Health (NIH)
Intramural Paper of the Month, NIEHS
2014
·
The National Institute of Environmental Health Sciences
Doctoral Dissertation Award
2010
·
The Scientific and Technological Research Council of Turkey
PhD visiting scholarship
2009
·
Yale Medical School
Teaching Profile
Courses Taught
2020-2021,2023-2024
BCH4905 Biochemistry Senior Research
2020-2024
BCH5413 Mammalian Molecular Biology and Genetics
2020-2021
HSC4970 Public Health and Health Professions Senior Honors Thesis
2018
MCB7979 Advanced Research
2021-2024
BCH6415 Advanced Molecular and Cell Biology
2022-2024
GMS6001 Fundamentals of Biomedical Sciences I
Research Profile
Open Researcher and Contributor ID (ORCID)
0000-0003-1107-1042
Publications
2024
Impact of polβ/XRCC1 Interaction Variants on the Efficiency of Nick Sealing by DNA Ligase IIIα in the Base Excision Repair Pathway
Journal of Molecular Biology.
436(4)
[DOI] 10.1016/j.jmb.2023.168410.
2024
Molecular Editing of NSC-666719 Enabling Discovery of benzodithiazinedioxide-guanidines as Anticancer Agents
RSC Medicinal Chemistry.
[DOI] 10.1039/d3md00648d.
2024
Structures of LIG1 provide a mechanistic basis for understanding a lack of sugar discrimination against a ribonucleotide at the 3′-end of nick DNA
Journal of Biological Chemistry.
300(5)
[DOI] 10.1016/j.jbc.2024.107216.
2024
Unfilled gaps by polβ lead to aberrant ligation by LIG1 at the downstream steps of base excision repair pathway
Nucleic Acids Research.
[DOI] 10.1093/nar/gkae104.
2022
Structures of LIG1 that engage with mutagenic mismatches inserted by polβ in base excision repair.
Nature communications.
13(1)
[DOI] 10.1038/s41467-022-31585-w.
[PMID] 35790757.
2021
DNA ligase I fidelity mediates the mutagenic ligation of pol β oxidized and mismatch nucleotide insertion products in base excision repair.
The Journal of biological chemistry.
296
[DOI] 10.1016/j.jbc.2021.100427.
[PMID] 33600799.
2021
The scaffold protein XRCC1 stabilizes the formation of polβ/gap DNA and ligase IIIα/nick DNA complexes in base excision repair.
The Journal of biological chemistry.
297(3)
[DOI] 10.1016/j.jbc.2021.101025.
[PMID] 34339737.
2020
DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage
Mutagenesis.
35(5):391-404
[DOI] 10.1093/mutage/geaa023.
[PMID] 32914844.
2020
Pol β gap filling, DNA ligation and substrate-product channeling during base excision repair opposite oxidized 5-methylcytosine modifications.
DNA repair.
95
[DOI] 10.1016/j.dnarep.2020.102945.
[PMID] 32853828.
2020
Pol μ ribonucleotide insertion opposite 8-oxodG facilitates the ligation of premutagenic DNA repair intermediate.
Scientific reports.
10(1)
[DOI] 10.1038/s41598-020-57886-y.
[PMID] 31969622.
2020
The ligation of pol β mismatch insertion products governs the formation of promutagenic base excision DNA repair intermediates
Nucleic Acids Research.
48(7):3708-3721
[DOI] 10.1093/nar/gkaa151.
[PMID] 32140717.
2019
Interplay between DNA Polymerases and DNA Ligases: Influence on Substrate Channeling and the Fidelity of DNA Ligation.
Journal of molecular biology.
431(11):2068-2081
[DOI] 10.1016/j.jmb.2019.04.028.
[PMID] 31034893.
2018
Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate.
Nature communications.
9(1)
[DOI] 10.1038/s41467-018-06700-5.
[PMID] 30310068.
2018
XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity.
DNA repair.
64:26-33
[DOI] 10.1016/j.dnarep.2018.02.004.
[PMID] 29477978.
2017
Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts.
Nucleic acids research.
45(17):10079-10088
[DOI] 10.1093/nar/gkx654.
[PMID] 28973450.
2017
DNA polymerase β: A missing link of the base excision repair machinery in mammalian mitochondria.
DNA repair.
60:77-88
[DOI] 10.1016/j.dnarep.2017.10.011.
[PMID] 29100041.
2017
Oxidized nucleotide insertion by pol β confounds ligation during base excision repair.
Nature communications.
8
[DOI] 10.1038/ncomms14045.
[PMID] 28067232.
2017
Role of DNA polymerase β oxidized nucleotide insertion in DNA ligation failure.
Journal of radiation research.
58(5):603-607
[DOI] 10.1093/jrr/rrx027.
[PMID] 28992331.
2016
Impact of Ribonucleotide Backbone on Translesion Synthesis and Repair of 7,8-Dihydro-8-oxoguanine.
The Journal of biological chemistry.
291(46):24314-24323
[PMID] 27660390.
2015
Complementation of aprataxin deficiency by base excision repair enzymes
Nucleic Acids Research.
43(4):2271-2281
[DOI] 10.1093/nar/gkv079.
[PMID] 25662216.
2015
Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair.
DNA repair.
35:85-9
[DOI] 10.1016/j.dnarep.2015.09.010.
[PMID] 26466358.
2014
Base excision repair of tandem modifications in a methylated CpG dinucleotide.
The Journal of biological chemistry.
289(20):13996-4008
[DOI] 10.1074/jbc.M114.557769.
[PMID] 24695738.
2014
Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair.
Nature structural & molecular biology.
21(5):497-9
[DOI] 10.1038/nsmb.2818.
[PMID] 24777061.
Grants
Aug 2022
ACTIVE
DNA ligase activities during base excision repair coordination
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS
Apr 2020
– Dec 2020
Structural and Mechanistic Studies of Base Excision DNA Repair
Role: Principal Investigator
Funding: UF FOUNDATION
Aug 2018
– Jul 2022
Oxidant and environmental toxicant-induced effects compromise ligation in DNA repair
Role: Principal Investigator
Funding: NATL INST OF HLTH NIEHS
Contact Details
Phones:
- Business:
- (352) 294-8383
Emails:
- Business:
- caglayanm@ufl.edu
Addresses:
- Business Mailing:
-
1200 NEWELL DRIVE ARB R3-228
GAINESVILLE FL 326100001