Brian D Cain

Brian D Cain,

PROFESSOR

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 294-8381
Business Email: bcain@ufl.edu

Teaching Profile

Courses Taught
2012-2020
BCH4024 Introduction to Biochemistry and Molecular Biology
2013-2021
BCH4905 Biochemistry Senior Research
2013-2015,2018-2020,2020
BCH6208 Advanced Metabolism: Regulation of Key Reactions in Carbohydrate and Lipid Metabolism
2013-2018,2020
BCH6206 Advanced Metabolism
2020
BCH6207 Advanced Metabolism: Role of Membranes in Signal Transduction and Metabolic Control
2016-2019
GMS5905 Special Topics in Biomedical Sciences
2012
BCH5413 Mammalian Molecular Biology and Genetics
2009
ALS4905 Problems in Agricul

Publications

2020
EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells.
Frontiers in physiology. 11 [DOI] 10.3389/fphys.2020.00209. [PMID] 32231591.
2020
Knockout of the circadian clock protein PER1 results in sex-dependent alterations of ET-1 production in mice in response to a high-salt diet plus mineralocorticoid treatment.
Canadian journal of physiology and pharmacology. 98(9):579-586 [DOI] 10.1139/cjpp-2019-0688. [PMID] 32437627.
2019
Female C57BL/6J mice lacking the circadian clock protein PER1 are protected from nondipping hypertension.
American journal of physiology. Regulatory, integrative and comparative physiology. 316(1):R50-R58 [DOI] 10.1152/ajpregu.00381.2017. [PMID] 30427705.
2018
Renal Na-handling defect associated with PER1-dependent nondipping hypertension in male mice.
American journal of physiology. Renal physiology. 314(6):F1138-F1144 [DOI] 10.1152/ajprenal.00546.2017. [PMID] 29357420.
2017
Desoxycorticosterone pivalate-salt treatment leads to non-dipping hypertension in Per1 knockout mice.
Acta physiologica (Oxford, England). 220(1):72-82 [DOI] 10.1111/apha.12804. [PMID] 27636900.
2016
Aldosterone alters the chromatin structure of the murine endothelin-1 gene.
Life sciences. 159:121-126 [DOI] 10.1016/j.lfs.2016.01.019. [PMID] 26775567.
2015
Effect of mineralocorticoid treatment in mice with collecting duct-specific knockout of endothelin-1.
American journal of physiology. Renal physiology. 309(12):F1026-34 [DOI] 10.1152/ajprenal.00220.2015. [PMID] 26400543.
2014
MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells.
Life sciences. 118(2):195-9 [DOI] 10.1016/j.lfs.2014.03.003. [PMID] 24632479.
2014
Tissue-specific and time-dependent regulation of the endothelin axis by the circadian clock protein Per1.
Life sciences. 118(2):255-62 [DOI] 10.1016/j.lfs.2014.03.028. [PMID] 24721511.
2013
An emerging role for microRNA in the regulation of endothelin-1.
Frontiers in physiology. 4 [DOI] 10.3389/fphys.2013.00022. [PMID] 23424003.
2013
Early progress in epigenetic regulation of endothelin pathway genes.
British journal of pharmacology. 168(2):327-34 [DOI] 10.1111/j.1476-5381.2012.01826.x. [PMID] 22220553.
2013
Endothelin-1 inhibits sodium reabsorption by ET(A) and ET(B) receptors in the mouse cortical collecting duct.
American journal of physiology. Renal physiology. 305(4):F568-73 [DOI] 10.1152/ajprenal.00613.2012. [PMID] 23698114.
2013
The Effect of Aldosterone On the Mirna Content of a Murine Inner Medullary Collecting Duct Cell
The FASEB's Journal. 27
2012
Dexamethasone stimulates endothelin-1 gene expression in renal collecting duct cells.
Steroids. 77(5):360-6 [DOI] 10.1016/j.steroids.2011.12.011. [PMID] 22209709.
2012
Evaluation of Enac and Fluid Secretion Along the Mouse Cd: Endothelin-1 Down-Regulates Na Reabsorption
The FASEB's Journal. 26
2012
The circadian protein period 1 contributes to blood pressure control and coordinately regulates renal sodium transport genes.
Hypertension (Dallas, Tex. : 1979). 59(6):1151-6 [DOI] 10.1161/HYPERTENSIONAHA.112.190892. [PMID] 22526258.
2011
Endothelin-1 gene regulation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 25(1):16-28 [DOI] 10.1096/fj.10-161612. [PMID] 20837776.
2011
Functional expression of human adenine nucleotide translocase 4 in Saccharomyces cerevisiae.
PloS one. 6(4) [DOI] 10.1371/journal.pone.0019250. [PMID] 21532989.
2011
Manipulations in the peripheral stalk of the Saccharomyces cerevisiae F1F0-ATP synthase.
The Journal of biological chemistry. 286(12):10155-62 [DOI] 10.1074/jbc.M110.213447. [PMID] 21257750.
2011
Mineralocorticoids stimulate the activity and expression of renal H+,K+-ATPases.
Journal of the American Society of Nephrology : JASN. 22(1):49-58 [DOI] 10.1681/ASN.2010030311. [PMID] 21164026.
2011
Two rotary motors in F-ATP synthase are elastically coupled by a flexible rotor and a stiff stator stalk.
Proceedings of the National Academy of Sciences of the United States of America. 108(10):3924-9 [DOI] 10.1073/pnas.1011581108. [PMID] 21368147.
2010
Pharmacological profiles of the murine gastric and colonic H,K-ATPases.
Biochimica et biophysica acta. 1800(9):906-11 [DOI] 10.1016/j.bbagen.2010.05.002. [PMID] 20594946.
2010
Regulation of ╬▒ENaC expression by the circadian clock protein Period 1 in mpkCCD(c14) cells.
Biochimica et biophysica acta. 1799(9):622-9 [DOI] 10.1016/j.bbagrm.2010.09.003. [PMID] 20868778.
2010
The renal H+-K+-ATPases: physiology, regulation, and structure.
American journal of physiology. Renal physiology. 298(1):F12-21 [DOI] 10.1152/ajprenal.90723.2008. [PMID] 19640897.
2010
The renal H,K-ATPases.
Current opinion in nephrology and hypertension. 19(5):478-82 [DOI] 10.1097/MNH.0b013e32833ce65f. [PMID] 20616716.
2009
Aldosterone modulates steroid receptor binding to the endothelin-1 gene (edn1).
The Journal of biological chemistry. 284(44):30087-96 [DOI] 10.1074/jbc.M109.030718. [PMID] 19638349.
2009
Basolateral expression of the ammonia transporter family member Rh C glycoprotein in the mouse kidney.
American journal of physiology. Renal physiology. 296(3):F543-55 [DOI] 10.1152/ajprenal.90637.2008. [PMID] 19129254.
2009
The b subunits in the peripheral stalk of F1F0 ATP synthase preferentially adopt an offset relationship.
The Journal of biological chemistry. 284(24):16531-16540 [DOI] 10.1074/jbc.M109.002980. [PMID] 19369253.
2009
The circadian clock protein Period 1 regulates expression of the renal epithelial sodium channel in mice.
The Journal of clinical investigation. 119(8):2423-34 [DOI] 10.1172/JCI36908. [PMID] 19587447.
2008
Changes in Crosslink Efficiency Between B Subunits in the Peripheral Stalk of F(1)F(O) Atp Synthase
Biochimica Et Biophysica Acta-Bioenergetics. 1777 [DOI] 10.1016/j.bbabio.2008.05.075.
2008
Expression Vectors for Mutant Peripheral Stalk Subunits in Escherichia Coli and Saccharomyces Cerevisiae
Biochimica Et Biophysica Acta-Bioenergetics. 1777 [DOI] 10.1016/j.bbabio.2008.05.076.
2008
Impaired acid secretion in cortical collecting duct intercalated cells from H-K-ATPase-deficient mice: role of HKalpha isoforms.
American journal of physiology. Renal physiology. 294(3):F621-7 [PMID] 18057185.
2008
The b (arg36) contributes to efficient coupling in F(1)F (O) ATP synthase in Escherichia coli.
Journal of bioenergetics and biomembranes. 40(1):1-8 [DOI] 10.1007/s10863-008-9124-3. [PMID] 18204891.
2007
Cellular distribution of the potassium channel KCNQ1 in normal mouse kidney.
American journal of physiology. Renal physiology. 292(1):F456-66 [PMID] 16896189.
2007
Functional incorporation of chimeric b subunits into F1Fo ATP synthase.
Journal of bacteriology. 189(15):5463-71 [PMID] 17526709.
2007
The renal H+, K+-ATPases as therapeutic targets.
Expert opinion on therapeutic targets. 11(7):881-90 [PMID] 17614757.
2006
Characterization of the rabbit HKalpha2 gene promoter.
Biochimica et biophysica acta. 1759(10):443-50 [PMID] 17034876.
2005
Manipulating the length of the b subunit F1 binding domain in F1F0 ATP synthase from Escherichia coli.
Journal of bioenergetics and biomembranes. 37(2):67-74 [PMID] 15906151.
2004
Genetic complementation between mutant b subunits in F1F0 ATP synthase.
The Journal of biological chemistry. 279(30):31205-11 [PMID] 15159387.
2003
Early transcriptional effects of aldosterone in a mouse inner medullary collecting duct cell line.
American journal of physiology. Renal physiology. 285(4):F664-73 [PMID] 12770840.
2003
Integration of b subunits of unequal lengths into F1F0-ATP synthase.
The Journal of biological chemistry. 278(37):34751-6 [PMID] 12842903.
2003
Molecular modeling of the rabbit colonic (HKalpha2a) H+, K+ ATPase.
Journal of molecular modeling. 9(5):283-9 [PMID] 12884085.
2003
Mutagenesis studies of the F1F0 ATP synthase b subunit membrane domain.
Journal of bioenergetics and biomembranes. 35(5):389-97 [PMID] 14740887.
2003
YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase.
Biochemistry. 42(35):10537-44 [PMID] 12950181.
2002
Molecular regulation of the HKalpha2 subunit of the H+,K(+)-ATPases.
Journal of nephrology. 15 Suppl 5:S54-60 [PMID] 12027222.
2001
Immunohistochemical localization of H-K-ATPase alpha(2c)-subunit in rabbit kidney.
American journal of physiology. Renal physiology. 281(2):F357-65 [PMID] 11457728.
2000
Mutagenic analysis of the F0 stator subunits.
Journal of bioenergetics and biomembranes. 32(4):365-71 [PMID] 11768298.
2000
The a subunit ala-217 –> arg substitution affects catalytic activity of F(1)F(0) ATP synthase.
Archives of biochemistry and biophysics. 380(1):201-7 [PMID] 10900150.
1999
Amino acid substitutions in the a subunit affect the epsilon subunit of F1F0 ATP synthase from Escherichia coli.
Archives of biochemistry and biophysics. 361(2):302-8 [PMID] 9882460.
1999
H-K-ATPase in the RCCT-28A rabbit cortical collecting duct cell line.
The American journal of physiology. 276(2):F237-45 [DOI] 10.1152/ajprenal.1999.276.2.F237. [PMID] 9950954.
1999
Lengthening the second stalk of F(1)F(0) ATP synthase in Escherichia coli.
The Journal of biological chemistry. 274(51):36261-6 [PMID] 10593914.
1999
Modeling the Leigh syndrome nt8993 T–>C mutation in Escherichia coli F1F0 ATP synthase.
The international journal of biochemistry & cell biology. 31(7):769-76 [PMID] 10467733.
1999
Molecular identification of the renal H+,K+-ATPases.
Seminars in nephrology. 19(5):431-7 [PMID] 10511383.
1998
Deletions in the second stalk of F1F0-ATP synthase in Escherichia coli.
The Journal of biological chemistry. 273(43):27873-8 [PMID] 9774398.
1998
Formation of the b subunit dimer is necessary for interaction with F1-ATPase.
Biochemistry. 37(3):923-32 [PMID] 9454582.
1998
Identification of an uncoupling mutation affecting the b subunit of F1F0 ATP synthase in Escherichia coli.
FEBS letters. 429(2):201-6 [PMID] 9650590.
1995
In situ hybridization of H-K-ATPase beta-subunit mRNA in rat and rabbit kidney.
The American journal of physiology. 269(3 Pt 2):F345-54 [PMID] 7573483.
1995
Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.
The American journal of physiology. 268(3 Pt 2):F363-74 [PMID] 7900835.
1994
Exploring the microtubule-binding region of bovine microtubule-associated protein-2 (MAP-2): cDNA sequencing, bacterial expression, and site-directed mutagenesis.
Biochemistry. 33(45):13199-207 [PMID] 7947727.
1994
Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli.
Journal of bacteriology. 176(2):540-2 [PMID] 8288552.
1994
Second-site suppressor mutations at glycine 218 and histidine 245 in the alpha subunit of F1F0 ATP synthase in Escherichia coli.
The Journal of biological chemistry. 269(51):32313-7 [PMID] 7798232.
1993
Amplification of the bacA gene confers bacitracin resistance to Escherichia coli.
Journal of bacteriology. 175(12):3784-9 [PMID] 8389741.
1993
Characterization of mutations in the b subunit of F1F0 ATP synthase in Escherichia coli.
The Journal of biological chemistry. 268(33):24683-91 [PMID] 8227028.
1993
Mutagenic analysis of the a subunit of the F1F0 ATP synthase in Escherichia coli: Gln-252 through Tyr-263.
Journal of bacteriology. 175(5):1337-43 [PMID] 8383111.
1993
The aleu207–>arg mutation in F1F0-ATP synthase from Escherichia coli. A model for human mitochondrial disease.
The Journal of biological chemistry. 268(17):12250-2 [PMID] 8509361.
1993
The renal H-K-ATPase: physiological significance and role in potassium homeostasis.
Annual review of physiology. 55:323-47 [PMID] 8385435.
1992
Evidence for the presence of a K-dependent acidifying adenosine triphosphatase in the rabbit renal medulla.
Kidney international. 42(5):1093-8 [PMID] 1333550.
1991
Targeted mutagenesis of the b subunit of F1F0 ATP synthase in Escherichia coli: Glu-77 through Gln-85.
Journal of bacteriology. 173(22):7240-8 [PMID] 1682301.
1989
Proton translocation by the F1F0ATPase of Escherichia coli. Mutagenic analysis of the a subunit.
The Journal of biological chemistry. 264(6):3292-300 [PMID] 2536742.
1988
Interaction between Glu-219 and His-245 within the a subunit of F1F0-ATPase in Escherichia coli.
The Journal of biological chemistry. 263(14):6606-12 [PMID] 2896197.
1988
Mutagenesis of the alpha subunit of the F1Fo-ATPase from Escherichia coli. Mutations at Glu-196, Pro-190, and Ser-199.
The Journal of biological chemistry. 263(14):6599-605 [PMID] 2896196.
1986
Impaired proton conductivity resulting from mutations in the a subunit of F1F0 ATPase in Escherichia coli.
The Journal of biological chemistry. 261(22):10043-50 [PMID] 2874137.
1984
Cell-cycle-specific biosynthesis of the photosynthetic membrane of Rhodopseudomonas sphaeroides. Structural implications.
Biochimica et biophysica acta. 777(1):41-55 [PMID] 6333251.
1983
In vivo metabolic intermediates of phospholipid biosynthesis in Rhodopseudomonas sphaeroides.
Journal of bacteriology. 156(1):375-85 [PMID] 6604726.
1982
Alterations in the phospholipid composition of Rhodopseudomonas sphaeroides and other bacteria induced by Tris.
Journal of bacteriology. 152(2):595-606 [PMID] 6982264.
1982
Kinetic analysis of N-acylphosphatidylserine accumulation and implications for membrane assembly in Rhodopseudomonas sphaeroides.
Journal of bacteriology. 152(2):607-15 [PMID] 6982265.
1982
Purification and characterization of an N-acylphosphatidylserine from Rhodopseudomonas sphaeroides.
Biochemistry. 21(11):2765-73 [PMID] 6980013.
1981
In vivo intermembrane transfer of phospholipids in the photosynthetic bacterium Rhodopseudomonas sphaeroides.
Journal of bacteriology. 145(3):1154-66 [PMID] 6970743.

Grants

Dec 2016 – Nov 2019
Novel mechanism to target the endothelin axis in aging and CKD
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
Sep 2009 – Aug 2015
An Aldosterone-Endothelin Feedback Mechanism on Sodium Homeostasis.
Role: Project Manager
Funding: NATL INST OF HLTH NIDDK

Contact Details

Phones:
Business:
(352) 294-8381
Emails:
Business:
bcain@ufl.edu
Addresses:
Business Mailing:
1200 NEWELL DR
GAINESVILLE FL 32610
Business Street:
R3254 ARB
GAINESVILLE FL 32611