Brian D Cain,
Professor Emeritus
Department:
MD-BIOCHEM / MOLECULAR BIOL
Business Phone:
(352) 294-8396
Business Email:
bcain@ufl.edu
About Brian D Cain
Professor Brian Cain earned his Ph.D. in Cell Biology from the University of Illinois in 1983. His graduate work focused on phospholipid metabolism. As a postdoctoral fellow at Stanford University, Dr. Cain began studying the F1F0 ATP synthase proton channel. In 1988, Dr. Cain joined the faculty of the University of Florida. He continued to work on the proton channel and showed plasticity of the enzyme’s peripheral stalk. In collaboration with Dr. Charles Wingo, Dr. Cain looked at expression of the renal H+,K+-ATPases and discovered that aldosterone stimulates endothelin-1 production in the kidney. Dr. Cain has served as Chair of the NIH Physical Biochemistry Study Section, Chair of the 2011 Bioenergetics Gordon Research Conference, and served two terms on the Editorial Board of The Journal of Biological Chemistry.
Teaching Profile
Courses Taught
2012-2020
BCH4024 Introduction to Biochemistry and Molecular Biology
2013-2021
BCH4905 Biochemistry Senior Research
2013-2015,2018-2020,2020
BCH6208 Advanced Metabolism: Regulation of Key Reactions in Carbohydrate and Lipid Metabolism
2013-2018,2020
BCH6206 Advanced Metabolism
2020
BCH6207 Advanced Metabolism: Role of Membranes in Signal Transduction and Metabolic Control
2016-2019
GMS5905 Special Topics in Biomedical Sciences
2012
BCH5413 Mammalian Molecular Biology and Genetics
2009
ALS4905 Problems in Agricul
Publications
Academic Articles
2023
Aldosterone: Renal Action and Physiological Effects.
Comprehensive Physiology.
13(2):4409-4491
[DOI] 10.1002/cphy.c190043.
[PMID] 36994769.
2020
EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells.
Frontiers in physiology.
11
[DOI] 10.3389/fphys.2020.00209.
[PMID] 32231591.
2020
Knockout of the circadian clock protein PER1 results in sex-dependent alterations of ET-1 production in mice in response to a high-salt diet plus mineralocorticoid treatment.
Canadian journal of physiology and pharmacology.
98(9):579-586
[DOI] 10.1139/cjpp-2019-0688.
[PMID] 32437627.
2019
Female C57BL/6J mice lacking the circadian clock protein PER1 are protected from nondipping hypertension.
American journal of physiology. Regulatory, integrative and comparative physiology.
316(1):R50-R58
[DOI] 10.1152/ajpregu.00381.2017.
[PMID] 30427705.
2018
Renal Na-handling defect associated with PER1-dependent nondipping hypertension in male mice.
American journal of physiology. Renal physiology.
314(6):F1138-F1144
[DOI] 10.1152/ajprenal.00546.2017.
[PMID] 29357420.
2017
Desoxycorticosterone pivalate-salt treatment leads to non-dipping hypertension in Per1 knockout mice.
Acta physiologica (Oxford, England).
220(1):72-82
[DOI] 10.1111/apha.12804.
[PMID] 27636900.
2016
Aldosterone alters the chromatin structure of the murine endothelin-1 gene.
Life sciences.
159:121-126
[DOI] 10.1016/j.lfs.2016.01.019.
[PMID] 26775567.
2015
Effect of mineralocorticoid treatment in mice with collecting duct-specific knockout of endothelin-1.
American journal of physiology. Renal physiology.
309(12):F1026-34
[DOI] 10.1152/ajprenal.00220.2015.
[PMID] 26400543.
2014
MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells.
Life sciences.
118(2):195-9
[DOI] 10.1016/j.lfs.2014.03.003.
[PMID] 24632479.
2014
Tissue-specific and time-dependent regulation of the endothelin axis by the circadian clock protein Per1.
Life sciences.
118(2):255-62
[DOI] 10.1016/j.lfs.2014.03.028.
[PMID] 24721511.
2013
An emerging role for microRNA in the regulation of endothelin-1.
Frontiers in physiology.
4
[DOI] 10.3389/fphys.2013.00022.
[PMID] 23424003.
2013
Early progress in epigenetic regulation of endothelin pathway genes.
British journal of pharmacology.
168(2):327-34
[DOI] 10.1111/j.1476-5381.2012.01826.x.
[PMID] 22220553.
2013
Endothelin-1 inhibits sodium reabsorption by ET(A) and ET(B) receptors in the mouse cortical collecting duct.
American journal of physiology. Renal physiology.
305(4):F568-73
[DOI] 10.1152/ajprenal.00613.2012.
[PMID] 23698114.
2013
The Effect of Aldosterone On the Mirna Content of a Murine Inner Medullary Collecting Duct Cell
The FASEB's Journal.
27
2012
Dexamethasone stimulates endothelin-1 gene expression in renal collecting duct cells.
Steroids.
77(5):360-6
[DOI] 10.1016/j.steroids.2011.12.011.
[PMID] 22209709.
2012
Evaluation of Enac and Fluid Secretion Along the Mouse Cd: Endothelin-1 Down-Regulates Na Reabsorption
The FASEB's Journal.
26
2012
The circadian protein period 1 contributes to blood pressure control and coordinately regulates renal sodium transport genes.
Hypertension (Dallas, Tex. : 1979).
59(6):1151-6
[DOI] 10.1161/HYPERTENSIONAHA.112.190892.
[PMID] 22526258.
2011
Endothelin-1 gene regulation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology.
25(1):16-28
[DOI] 10.1096/fj.10-161612.
[PMID] 20837776.
2011
Functional expression of human adenine nucleotide translocase 4 in Saccharomyces cerevisiae.
PloS one.
6(4)
[DOI] 10.1371/journal.pone.0019250.
[PMID] 21532989.
2011
Manipulations in the peripheral stalk of the Saccharomyces cerevisiae F1F0-ATP synthase.
The Journal of biological chemistry.
286(12):10155-62
[DOI] 10.1074/jbc.M110.213447.
[PMID] 21257750.
2011
Mineralocorticoids stimulate the activity and expression of renal H+,K+-ATPases.
Journal of the American Society of Nephrology : JASN.
22(1):49-58
[DOI] 10.1681/ASN.2010030311.
[PMID] 21164026.
2011
Two rotary motors in F-ATP synthase are elastically coupled by a flexible rotor and a stiff stator stalk.
Proceedings of the National Academy of Sciences of the United States of America.
108(10):3924-9
[DOI] 10.1073/pnas.1011581108.
[PMID] 21368147.
2010
Pharmacological profiles of the murine gastric and colonic H,K-ATPases.
Biochimica et biophysica acta.
1800(9):906-11
[DOI] 10.1016/j.bbagen.2010.05.002.
[PMID] 20594946.
2010
Regulation of αENaC expression by the circadian clock protein Period 1 in mpkCCD(c14) cells.
Biochimica et biophysica acta.
1799(9):622-9
[DOI] 10.1016/j.bbagrm.2010.09.003.
[PMID] 20868778.
2010
The renal H+-K+-ATPases: physiology, regulation, and structure.
American journal of physiology. Renal physiology.
298(1):F12-21
[DOI] 10.1152/ajprenal.90723.2008.
[PMID] 19640897.
2010
The renal H,K-ATPases.
Current opinion in nephrology and hypertension.
19(5):478-82
[DOI] 10.1097/MNH.0b013e32833ce65f.
[PMID] 20616716.
2009
Aldosterone modulates steroid receptor binding to the endothelin-1 gene (edn1).
The Journal of biological chemistry.
284(44):30087-96
[DOI] 10.1074/jbc.M109.030718.
[PMID] 19638349.
2009
Basolateral expression of the ammonia transporter family member Rh C glycoprotein in the mouse kidney.
American journal of physiology. Renal physiology.
296(3):F543-55
[DOI] 10.1152/ajprenal.90637.2008.
[PMID] 19129254.
2009
The b subunits in the peripheral stalk of F1F0 ATP synthase preferentially adopt an offset relationship.
The Journal of biological chemistry.
284(24):16531-16540
[DOI] 10.1074/jbc.M109.002980.
[PMID] 19369253.
2009
The circadian clock protein Period 1 regulates expression of the renal epithelial sodium channel in mice.
The Journal of clinical investigation.
119(8):2423-34
[DOI] 10.1172/JCI36908.
[PMID] 19587447.
2008
Changes in Crosslink Efficiency Between B Subunits in the Peripheral Stalk of F(1)F(O) Atp Synthase
Biochimica Et Biophysica Acta-Bioenergetics.
1777
[DOI] 10.1016/j.bbabio.2008.05.075.
2008
Expression Vectors for Mutant Peripheral Stalk Subunits in Escherichia Coli and Saccharomyces Cerevisiae
Biochimica Et Biophysica Acta-Bioenergetics.
1777
[DOI] 10.1016/j.bbabio.2008.05.076.
2008
Impaired acid secretion in cortical collecting duct intercalated cells from H-K-ATPase-deficient mice: role of HKalpha isoforms.
American journal of physiology. Renal physiology.
294(3):F621-7
[PMID] 18057185.
2008
The b (arg36) contributes to efficient coupling in F(1)F (O) ATP synthase in Escherichia coli.
Journal of bioenergetics and biomembranes.
40(1):1-8
[DOI] 10.1007/s10863-008-9124-3.
[PMID] 18204891.
2007
Cellular distribution of the potassium channel KCNQ1 in normal mouse kidney.
American journal of physiology. Renal physiology.
292(1):F456-66
[PMID] 16896189.
2007
Functional incorporation of chimeric b subunits into F1Fo ATP synthase.
Journal of bacteriology.
189(15):5463-71
[PMID] 17526709.
2007
The renal H+, K+-ATPases as therapeutic targets.
Expert opinion on therapeutic targets.
11(7):881-90
[PMID] 17614757.
2006
Characterization of the rabbit HKalpha2 gene promoter.
Biochimica et biophysica acta.
1759(10):443-50
[PMID] 17034876.
2005
Manipulating the length of the b subunit F1 binding domain in F1F0 ATP synthase from Escherichia coli.
Journal of bioenergetics and biomembranes.
37(2):67-74
[PMID] 15906151.
2004
Genetic complementation between mutant b subunits in F1F0 ATP synthase.
The Journal of biological chemistry.
279(30):31205-11
[PMID] 15159387.
2003
Early transcriptional effects of aldosterone in a mouse inner medullary collecting duct cell line.
American journal of physiology. Renal physiology.
285(4):F664-73
[PMID] 12770840.
2003
Integration of b subunits of unequal lengths into F1F0-ATP synthase.
The Journal of biological chemistry.
278(37):34751-6
[PMID] 12842903.
2003
Molecular modeling of the rabbit colonic (HKalpha2a) H+, K+ ATPase.
Journal of molecular modeling.
9(5):283-9
[PMID] 12884085.
2003
Mutagenesis studies of the F1F0 ATP synthase b subunit membrane domain.
Journal of bioenergetics and biomembranes.
35(5):389-97
[PMID] 14740887.
2003
YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase.
Biochemistry.
42(35):10537-44
[PMID] 12950181.
2002
Molecular regulation of the HKalpha2 subunit of the H+,K(+)-ATPases.
Journal of nephrology.
15 Suppl 5:S54-60
[PMID] 12027222.
2001
Immunohistochemical localization of H-K-ATPase alpha(2c)-subunit in rabbit kidney.
American journal of physiology. Renal physiology.
281(2):F357-65
[PMID] 11457728.
2000
Mutagenic analysis of the F0 stator subunits.
Journal of bioenergetics and biomembranes.
32(4):365-71
[PMID] 11768298.
2000
The a subunit ala-217 –> arg substitution affects catalytic activity of F(1)F(0) ATP synthase.
Archives of biochemistry and biophysics.
380(1):201-7
[PMID] 10900150.
1999
Amino acid substitutions in the a subunit affect the epsilon subunit of F1F0 ATP synthase from Escherichia coli.
Archives of biochemistry and biophysics.
361(2):302-8
[PMID] 9882460.
1999
H-K-ATPase in the RCCT-28A rabbit cortical collecting duct cell line.
The American journal of physiology.
276(2):F237-45
[DOI] 10.1152/ajprenal.1999.276.2.F237.
[PMID] 9950954.
1999
Lengthening the second stalk of F(1)F(0) ATP synthase in Escherichia coli.
The Journal of biological chemistry.
274(51):36261-6
[PMID] 10593914.
1999
Modeling the Leigh syndrome nt8993 T–>C mutation in Escherichia coli F1F0 ATP synthase.
The international journal of biochemistry & cell biology.
31(7):769-76
[PMID] 10467733.
1999
Molecular identification of the renal H+,K+-ATPases.
Seminars in nephrology.
19(5):431-7
[PMID] 10511383.
1998
Deletions in the second stalk of F1F0-ATP synthase in Escherichia coli.
The Journal of biological chemistry.
273(43):27873-8
[PMID] 9774398.
1998
Formation of the b subunit dimer is necessary for interaction with F1-ATPase.
Biochemistry.
37(3):923-32
[PMID] 9454582.
1998
Identification of an uncoupling mutation affecting the b subunit of F1F0 ATP synthase in Escherichia coli.
FEBS letters.
429(2):201-6
[PMID] 9650590.
1995
In situ hybridization of H-K-ATPase beta-subunit mRNA in rat and rabbit kidney.
The American journal of physiology.
269(3 Pt 2):F345-54
[PMID] 7573483.
1995
Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.
The American journal of physiology.
268(3 Pt 2):F363-74
[PMID] 7900835.
1994
Exploring the microtubule-binding region of bovine microtubule-associated protein-2 (MAP-2): cDNA sequencing, bacterial expression, and site-directed mutagenesis.
Biochemistry.
33(45):13199-207
[PMID] 7947727.
1994
Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli.
Journal of bacteriology.
176(2):540-2
[PMID] 8288552.
1994
Second-site suppressor mutations at glycine 218 and histidine 245 in the alpha subunit of F1F0 ATP synthase in Escherichia coli.
The Journal of biological chemistry.
269(51):32313-7
[PMID] 7798232.
1993
Amplification of the bacA gene confers bacitracin resistance to Escherichia coli.
Journal of bacteriology.
175(12):3784-9
[PMID] 8389741.
1993
Characterization of mutations in the b subunit of F1F0 ATP synthase in Escherichia coli.
The Journal of biological chemistry.
268(33):24683-91
[PMID] 8227028.
1993
Mutagenic analysis of the a subunit of the F1F0 ATP synthase in Escherichia coli: Gln-252 through Tyr-263.
Journal of bacteriology.
175(5):1337-43
[PMID] 8383111.
1993
The aleu207–>arg mutation in F1F0-ATP synthase from Escherichia coli. A model for human mitochondrial disease.
The Journal of biological chemistry.
268(17):12250-2
[PMID] 8509361.
1993
The renal H-K-ATPase: physiological significance and role in potassium homeostasis.
Annual review of physiology.
55:323-47
[PMID] 8385435.
1992
Evidence for the presence of a K-dependent acidifying adenosine triphosphatase in the rabbit renal medulla.
Kidney international.
42(5):1093-8
[PMID] 1333550.
1991
Targeted mutagenesis of the b subunit of F1F0 ATP synthase in Escherichia coli: Glu-77 through Gln-85.
Journal of bacteriology.
173(22):7240-8
[PMID] 1682301.
1989
Proton translocation by the F1F0ATPase of Escherichia coli. Mutagenic analysis of the a subunit.
The Journal of biological chemistry.
264(6):3292-300
[PMID] 2536742.
1988
Interaction between Glu-219 and His-245 within the a subunit of F1F0-ATPase in Escherichia coli.
The Journal of biological chemistry.
263(14):6606-12
[PMID] 2896197.
1988
Mutagenesis of the alpha subunit of the F1Fo-ATPase from Escherichia coli. Mutations at Glu-196, Pro-190, and Ser-199.
The Journal of biological chemistry.
263(14):6599-605
[PMID] 2896196.
1986
Impaired proton conductivity resulting from mutations in the a subunit of F1F0 ATPase in Escherichia coli.
The Journal of biological chemistry.
261(22):10043-50
[PMID] 2874137.
1984
Cell-cycle-specific biosynthesis of the photosynthetic membrane of Rhodopseudomonas sphaeroides. Structural implications.
Biochimica et biophysica acta.
777(1):41-55
[PMID] 6333251.
1983
In vivo metabolic intermediates of phospholipid biosynthesis in Rhodopseudomonas sphaeroides.
Journal of bacteriology.
156(1):375-85
[PMID] 6604726.
1982
Alterations in the phospholipid composition of Rhodopseudomonas sphaeroides and other bacteria induced by Tris.
Journal of bacteriology.
152(2):595-606
[PMID] 6982264.
1982
Kinetic analysis of N-acylphosphatidylserine accumulation and implications for membrane assembly in Rhodopseudomonas sphaeroides.
Journal of bacteriology.
152(2):607-15
[PMID] 6982265.
1982
Purification and characterization of an N-acylphosphatidylserine from Rhodopseudomonas sphaeroides.
Biochemistry.
21(11):2765-73
[PMID] 6980013.
1981
In vivo intermembrane transfer of phospholipids in the photosynthetic bacterium Rhodopseudomonas sphaeroides.
Journal of bacteriology.
145(3):1154-66
[PMID] 6970743.
Grants
Dec 2016
– Nov 2019
Novel mechanism to target the endothelin axis in aging and CKD
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
Sep 2009
– Aug 2015
An Aldosterone-Endothelin Feedback Mechanism on Sodium Homeostasis.
Role: Project Manager
Funding: NATL INST OF HLTH NIDDK
Contact Details
Phones:
- Business:
- (352) 294-8396
Emails:
- Business:
- bcain@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100245
GAINESVILLE FL 32610 - Business Street:
-
ARB, R3-234
1200 Newell Dr
GAINESVILLE FL 32610