James B Flanegan

James B Flanegan,

Professor

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 294-8384
Business Email: flanegan@ufl.edu

Teaching Profile

Courses Taught
2013-2017,2019-2022
GMS6034 Advanced Virology I: Genetics and RNA
2013-2017,2019-2022
GMS6035 Advanced Virology II: RNA Viruses
2014-2019,2021-2022
BCH6415 Advanced Molecular and Cell Biology
2018
GMS7980 Research for Doctoral Dissertation
2018
GMS7979 Advanced Research
2015
BCH4905 Biochemistry Senior Research

Publications

2015
Viral precursor protein P3 and its processed products perform discrete and essential functions in the poliovirus RNA replication complex.
Virology. 485:492-501 [DOI] 10.1016/j.virol.2015.07.018. [PMID] 26303005.
2014
Amiloride inhibits the initiation of Coxsackievirus and poliovirus RNA replication by inhibiting VPg uridylylation.
Virology. 464-465:87-97 [DOI] 10.1016/j.virol.2014.06.025. [PMID] 25058507.
2011
Non-template functions of viral RNA in picornavirus replication.
Current opinion in virology. 1(5):339-46 [DOI] 10.1016/j.coviro.2011.09.005. [PMID] 22140418.
2010
The 5’CL-PCBP RNP complex, 3′ poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA.
Virology. 397(1):14-22 [DOI] 10.1016/j.virol.2009.11.006. [PMID] 19945132.
2009
Functional role of the 5′ terminal cloverleaf in Coxsackievirus RNA replication.
Virology. 393(2):238-49 [DOI] 10.1016/j.virol.2009.07.039. [PMID] 19732932.
2008
Protein-RNA tethering: the role of poly(C) binding protein 2 in poliovirus RNA replication.
Virology. 374(2):280-91 [DOI] 10.1016/j.virol.2007.12.039. [PMID] 18252259.
2006
2Apro is a multifunctional protein that regulates the stability, translation and replication of poliovirus RNA.
Virology. 345(2):346-57 [PMID] 16297952.
2006
Relationship between poliovirus negative-strand RNA synthesis and the length of the 3′ poly(A) tail.
Virology. 345(2):509-19 [PMID] 16297425.
2005
3′-Terminal sequence in poliovirus negative-strand templates is the primary cis-acting element required for VPgpUpU-primed positive-strand initiation.
Journal of virology. 79(6):3565-77 [PMID] 15731251.
2004
Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis of acute myeloid leukemia.
Proceedings of the National Academy of Sciences of the United States of America. 101(14):4924-9 [PMID] 15044690.
2003
Initiation of poliovirus negative-strand RNA synthesis requires precursor forms of p2 proteins.
Journal of virology. 77(2):1075-83 [PMID] 12502823.
2003
Poliovirus cre(2C)-dependent synthesis of VPgpUpU is required for positive- but not negative-strand RNA synthesis.
Journal of virology. 77(9):5136-44 [PMID] 12692216.
2001
5′ cloverleaf in poliovirus RNA is a cis-acting replication element required for negative-strand synthesis.
The EMBO journal. 20(6):1439-48 [PMID] 11250909.
1999
Translating ribosomes inhibit poliovirus negative-strand RNA synthesis.
Journal of virology. 73(12):10104-12 [PMID] 10559325.
1997
Poliovirus RNA recombination in cell-free extracts.
RNA (New York, N.Y.). 3(6):624-33 [PMID] 9174097.
1997
Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C.
Journal of virology. 71(11):8482-9 [PMID] 9343205.
1996
Assays for poliovirus polymerase, 3D(Pol), and authentic RNA replication in HeLa S10 extracts.
Methods in enzymology. 275:35-57 [PMID] 9026649.
1996
Poliovirus RNA polymerase mutation 3D-M394T results in a temperature-sensitive defect in RNA synthesis.
Virology. 217(2):459-69 [PMID] 8610437.
1995
Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
Journal of virology. 69(9):5516-27 [PMID] 7636997.
1993
Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus.
Journal of virology. 67(2):822-31 [PMID] 8380467.
1992
Determination of the poliovirus RNA polymerase error frequency at eight sites in the viral genome.
Journal of virology. 66(6):3784-93 [PMID] 1316481.
1992
Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells.
Journal of virology. 66(11):6480-8 [PMID] 1328676.
1989
A conserved base pair within helix P4 of the Tetrahymena ribozyme helps to form the tertiary structure required for self-splicing.
The EMBO journal. 8(11):3391-9 [PMID] 2684642.
1989
A point mutation in the poliovirus polymerase gene determines a complementable temperature-sensitive defect of RNA replication.
Virology. 168(2):302-11 [PMID] 2536984.
1989
Interference with vaccinia virus growth caused by insertion of the coding sequence for poliovirus protease 2A.
Virology. 173(2):509-21 [PMID] 2556841.
1989
Self-catalyzed linkage of poliovirus terminal protein VPg to poliovirus RNA.
Cell. 59(3):511-9 [PMID] 2553272.
1988
Direct measurement of the poliovirus RNA polymerase error frequency in vitro.
Journal of virology. 62(2):558-62 [PMID] 2826815.
1988
Measurement of poliovirus RNA polymerase binding to poliovirion and nonviral RNAs using a filter-binding assay.
Nucleic acids research. 16(21):10339-52 [PMID] 2461545.
1987
Characterization of product RNAs synthesized in vitro by poliovirus RNA polymerase purified by chromatography on hydroxylapatite or poly(U) Sepharose.
Journal of virology. 61(2):611-4 [PMID] 3027390.
1986
Anti-VPg antibody precipitation of product RNA synthesized in vitro by the poliovirus polymerase and host factor is mediated by VPg on the poliovirion RNA template.
Journal of virology. 58(3):715-23 [PMID] 3009884.
1985
Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro.
Journal of virology. 54(2):256-64 [PMID] 2985794.
1982
Genome-length copies of poliovirion RNA are synthesized in vitro by the poliovirus RNA-dependent RNA polymerase.
The Journal of biological chemistry. 257(8):4610-7 [PMID] 6279640.
1982
Poliovirus RNA-dependent RNA polymerase synthesizes full-length copies of poliovirion RNA, cellular mRNA, and several plant virus RNAs in vitro.
Journal of virology. 44(1):209-16 [PMID] 6183446.
1980
Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.
Journal of virology. 35(3):732-40 [PMID] 6252335.
1979
Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.
Journal of virology. 32(1):155-61 [PMID] 232168.
1979
Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.
Journal of virology. 29(1):352-60 [PMID] 219230.
1977
5′-Terminal nucleotide sequences of polio virus polyribosomal RNA and virion RNA are identical.
Nature. 268(5617):270-2 [PMID] 196211.
1977
Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus.
Proceedings of the National Academy of Sciences of the United States of America. 74(3):961-5 [PMID] 191841.
1977
Inhibitory effect of agents altering the structure of DNA on the synthesis of pyrimidine deoxyribonucleotides in bacteriophage T4 DNA replication.
The Journal of biological chemistry. 252(17):6031-7 [PMID] 330522.
1977
Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).
Proceedings of the National Academy of Sciences of the United States of America. 74(9):3677-80 [PMID] 198796.
1977
Regulation of deoxyribonucleotide biosynthesis during in vivo bacteriophage T4 DNA replication. Intrinsic control of synthesis of thymine and 5-hydroxymethylcytosine deoxyribonucleotides at precise ratio found in DNA.
The Journal of biological chemistry. 252(9):3019-27 [PMID] 323259.
1977
Role of deoxycytidylate deaminase in deoxyribonucleotide synthesis in bacteriophage T4 DNA replication.
The Journal of biological chemistry. 252(23):8603-8 [PMID] 925015.
1973
Noncovalent interactions in viruses: characterization of their role in the pH and thermally induced conformational changes in bromegrass mosaic virus.
Virology. 53(1):204-14 [PMID] 4706709.
1971
Measurement of temperature within the sample cell during sedimentation velocity experiments.
Analytical biochemistry. 40(2):267-80 [PMID] 5551540.

Grants

Jan 2020 – May 2021
Improving the activity of diamidines for potential therapeutic use for patients with myotonic dystrophy types 1 and 2
Role: Other
Funding: MYOTONIC DYSTROPHY FOU
Jan 2020 – Mar 2021
Improving the activity of diamidines for potential therapeutic use for patients with myotonic dystrophy types 1 and 2
Role: Other
Funding: MYOTONIC DYSTROPHY FOU
Feb 2019 – Dec 2021
Generation of Virus-Free Polio Vaccine Project: Phase IV, To progress Recombinant Poliovirus Virus-Like Particles (VLPs) into Commercially Viable Candidates
Role: Principal Investigator
Funding: UNIVERSITY OF LEEDS via WORLD HLTH ORGANIZATION
Jan 2018 – Dec 2018
Characterization of Poliovirus VLPs Using a Mammalian Cell-Free System
Role: Principal Investigator
Funding: WORLD HLTH ORGANIZATION
Mar 2017 – Dec 2017
Characterization of Poliovirus VLPs Using a Mammalian Cell-Free System
Role: Principal Investigator
Funding: WORLD HLTH ORGANIZATION
Jul 2015 – Jun 2016
IPA AGREEMENT
Role: Principal Investigator
Funding: US DEPT OF VET AFF GAINESVILLE MED CTR
Apr 2014 – Mar 2017
IPA AGREEMENT
Role: Principal Investigator
Funding: US DEPT OF VET AFF GAINESVILLE MED CTR
Oct 2013 – Sep 2016
IPA for Brian Cain
Role: Principal Investigator
Funding: US DEPT OF VETERANS AFFAIRS

Contact Details

Phones:
Business:
(352) 294-8384
Emails:
Business:
flanegan@ufl.edu
Addresses:
Business Mailing:
PO Box 100245
GAINESVILLE FL 32610
Business Street:
CAMPUS BOX 100245
1600 SW ARCHER RD
R3-234A ARB
GAINESVILLE FL 326103003