Michael S Kilberg,
Department: MD-BIOCHEM / MOLECULAR BIOL
Business Email: firstname.lastname@example.org
BMS6031 Foundations of Med
DEN5121 Biochm Molec Cell Bio
BCH7410 Advanced Gene Regulation
GMS7980 Research for Doctoral Dissertation
GMS7979 Advanced Research
Characterizing asparagine synthetase deficiency variants in lymphoblastoid cell lines.
JIMD reports. 64(2):167-179 [DOI] 10.1002/jmd2.12356. [PMID] 36873094.
Metabolomic Profiling of Asparagine Deprivation in Asparagine Synthetase Deficiency Patient-Derived Cells.
Nutrients. 15(8) [DOI] 10.3390/nu15081938. [PMID] 37111157.
Analysis of Enzyme Activity and Cellular Function for the N80S and S480F Asparagine Synthetase Variants Expressed in a Child with Asparagine Synthetase Deficiency.
International journal of molecular sciences. 24(1) [DOI] 10.3390/ijms24010559. [PMID] 36613999.
Cellular and molecular characterization of two novel asparagine synthetase gene mutations linked to asparagine synthetase deficiency.
The Journal of biological chemistry. 298(9) [DOI] 10.1016/j.jbc.2022.102385. [PMID] 35985424.
Sequencing of Argonaute-bound microRNA/mRNA hybrids reveals regulation of the unfolded protein response by microRNA-320a.
PLoS genetics. 17(12) [DOI] 10.1371/journal.pgen.1009934. [PMID] 34914716.
Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling.
Cellular and molecular gastroenterology and hepatology. 9(1):1-13 [DOI] 10.1016/j.jcmgh.2019.08.003. [PMID] 31421261.
Myeloma Cells Deplete Bone Marrow Glutamine and Inhibit Osteoblast Differentiation Limiting Asparagine Availability.
Cancers. 12(11) [DOI] 10.3390/cancers12113267. [PMID] 33167336.
PACT-mediated PKR activation acts as a hyperosmotic stress intensity sensor weakening osmoadaptation and enhancing inflammation.
eLife. 9 [DOI] 10.7554/eLife.52241. [PMID] 32175843.
The role of asparagine synthetase on nutrient metabolism in pancreatic disease.
Pancreatology : official journal of the International Association of Pancreatology (IAP) … [et al.]. 20(6):1029-1034 [DOI] 10.1016/j.pan.2020.08.002. [PMID] 32800652.
Asparagine Synthetase in Cancer: Beyond Acute Lymphoblastic Leukemia.
Frontiers in oncology. 9 [DOI] 10.3389/fonc.2019.01480. [PMID] 31998641.
Induction of early growth response gene 1 (EGR1) by endoplasmic reticulum stress is mediated by the extracellular regulated kinase (ERK) arm of the MAPK pathways.
Biochimica et biophysica acta. Molecular cell research. 1866(3):371-381 [DOI] 10.1016/j.bbamcr.2018.09.009. [PMID] 30290239.
Characterization of a novel variant in siblings with Asparagine Synthetase Deficiency.
Molecular genetics and metabolism. 123(3):317-325 [DOI] 10.1016/j.ymgme.2017.12.433. [PMID] 29279279.
Regulation of the ATF3 gene by a single promoter in response to amino acid availability and endoplasmic reticulum stress in human primary hepatocytes and hepatoma cells.
Biochimica et biophysica acta. Gene regulatory mechanisms. 1861(2):72-79 [DOI] 10.1016/j.bbagrm.2018.01.002. [PMID] 29413899.
Asparagine synthetase: Function, structure, and role in disease.
The Journal of biological chemistry. 292(49):19952-19958 [DOI] 10.1074/jbc.R117.819060. [PMID] 29084849.
Disulfide bond disrupting agents activate the unfolded protein response in EGFR- and HER2-positive breast tumor cells.
Oncotarget. 8(17):28971-28989 [DOI] 10.18632/oncotarget.15952. [PMID] 28423644.
Surviving Stress: Modulation of ATF4-Mediated Stress Responses in Normal and Malignant Cells.
Trends in endocrinology and metabolism: TEM. 28(11):794-806 [DOI] 10.1016/j.tem.2017.07.003. [PMID] 28797581.
Influence of Amino Acid Metabolism on Embryonic Stem Cell Function and Differentiation.
Advances in nutrition (Bethesda, Md.). 7(4):780S-9S [DOI] 10.3945/an.115.011031. [PMID] 27422515.
The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation.
Nucleic acids research. 44(20):9719-9732 [PMID] 27471030.
Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress.
Oncotarget. 7(3):3128-43 [DOI] 10.18632/oncotarget.6519. [PMID] 26657730.
Asparagine Synthetase Deficiency causes reduced proliferation of cells under conditions of limited asparagine.
Molecular genetics and metabolism. 116(3):178-86 [DOI] 10.1016/j.ymgme.2015.08.007. [PMID] 26318253.
HIF1α Represses Cell Stress Pathways to Allow Proliferation of Hypoxic Fetal Cardiomyocytes.
Developmental cell. 33(5):507-21 [DOI] 10.1016/j.devcel.2015.04.021. [PMID] 26028220.
Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element.
The Journal of biological chemistry. 290(25):15878-15891 [DOI] 10.1074/jbc.M114.635144. [PMID] 25931127.
MAPK signaling triggers transcriptional induction of cFOS during amino acid limitation of HepG2 cells.
Biochimica et biophysica acta. 1853(3):539-48 [DOI] 10.1016/j.bbamcr.2014.12.013. [PMID] 25523140.
A mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-dependent transcriptional program controls activation of the early growth response 1 (EGR1) gene during amino acid limitation.
The Journal of biological chemistry. 289(35):24665-79 [DOI] 10.1074/jbc.M114.565028. [PMID] 25028509.
Genomic and proteomic analysis of transcription factor TFII-I reveals insight into the response to cellular stress.
Nucleic acids research. 42(12):7625-41 [DOI] 10.1093/nar/gku467. [PMID] 24875474.
Activation of the amino acid response modulates lineage specification during differentiation of murine embryonic stem cells.
American journal of physiology. Endocrinology and metabolism. 305(3):E325-35 [DOI] 10.1152/ajpendo.00136.2013. [PMID] 23736538.
Asparagine synthetase: regulation by cell stress and involvement in tumor biology.
American journal of physiology. Endocrinology and metabolism. 304(8):E789-99 [DOI] 10.1152/ajpendo.00015.2013. [PMID] 23403946.
CHOP induces activating transcription factor 5 (ATF5) to trigger apoptosis in response to perturbations in protein homeostasis.
Molecular biology of the cell. 24(15):2477-90 [DOI] 10.1091/mbc.E13-01-0067. [PMID] 23761072.
Dynamic changes in genomic histone association and modification during activation of the ASNS and ATF3 genes by amino acid limitation.
The Biochemical journal. 449(1):219-29 [DOI] 10.1042/BJ20120958. [PMID] 22978410.
Elevated cJUN expression and an ATF/CRE site within the ATF3 promoter contribute to activation of ATF3 transcription by the amino acid response.
Physiological genomics. 45(4):127-37 [DOI] 10.1152/physiolgenomics.00160.2012. [PMID] 23269699.
ER-stress-induced transcriptional regulation increases protein synthesis leading to cell death.
Nature cell biology. 15(5):481-90 [DOI] 10.1038/ncb2738. [PMID] 23624402.
ATF4-dependent regulation of the JMJD3 gene during amino acid deprivation can be rescued in Atf4-deficient cells by inhibition of deacetylation.
The Journal of biological chemistry. 287(43):36393-403 [DOI] 10.1074/jbc.M112.399600. [PMID] 22955275.
The transcription factor network associated with the amino acid response in mammalian cells.
Advances in nutrition (Bethesda, Md.). 3(3):295-306 [DOI] 10.3945/an.112.001891. [PMID] 22585903.
Auto-activation of c-JUN gene by amino acid deprivation of hepatocellular carcinoma cells reveals a novel c-JUN-mediated signaling pathway.
The Journal of biological chemistry. 286(42):36724-38 [DOI] 10.1074/jbc.M111.277673. [PMID] 21862593.
Parkin is transcriptionally regulated by ATF4: evidence for an interconnection between mitochondrial stress and ER stress.
Cell death and differentiation. 18(5):769-82 [DOI] 10.1038/cdd.2010.142. [PMID] 21113145.
Expression profiling after activation of amino acid deprivation response in HepG2 human hepatoma cells.
Physiological genomics. 41(3):315-27 [DOI] 10.1152/physiolgenomics.00217.2009. [PMID] 20215415.
ATF4-dependent transcription mediates signaling of amino acid limitation.
Trends in endocrinology and metabolism: TEM. 20(9):436-43 [DOI] 10.1016/j.tem.2009.05.008. [PMID] 19800252.
Elevated ATF4 expression, in the absence of other signals, is sufficient for transcriptional induction via CCAAT enhancer-binding protein-activating transcription factor response elements.
The Journal of biological chemistry. 284(32):21241-8 [DOI] 10.1074/jbc.M109.011338. [PMID] 19509279.
Protein or amino acid deprivation differentially regulates the hepatic forkhead box protein A (FOXA) genes through an activating transcription factor-4-independent pathway.
Hepatology (Baltimore, Md.). 50(1):282-90 [DOI] 10.1002/hep.22971. [PMID] 19415718.
Specificity of amino acid regulated gene expression: analysis of genes subjected to either complete or single amino acid deprivation.
Amino acids. 37(1):79-88 [DOI] 10.1007/s00726-008-0199-2. [PMID] 19009228.
Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the ATF6 and IRE1/XBP1 arms of the pathway.
The Biochemical journal. 417(3):695-703 [DOI] 10.1042/BJ20081706. [PMID] 18840095.
Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex.
Nucleic acids research. 36(17):5571-80 [DOI] 10.1093/nar/gkn538. [PMID] 18757893.
C/EBP homology protein (CHOP) interacts with activating transcription factor 4 (ATF4) and negatively regulates the stress-dependent induction of the asparagine synthetase gene.
The Journal of biological chemistry. 283(50):35106-17 [DOI] 10.1074/jbc.M806874200. [PMID] 18940792.
Correlation between asparaginase sensitivity and asparagine synthetase protein content, but not mRNA, in acute lymphoblastic leukemia cell lines.
Pediatric blood & cancer. 50(2):274-9 [PMID] 17514734.
Deprivation of protein or amino acid induces C/EBPbeta synthesis and binding to amino acid response elements, but its action is not an absolute requirement for enhanced transcription.
The Biochemical journal. 410(3):473-84 [PMID] 18052938.
Despite increased ATF4 binding at the C/EBP-ATF composite site following activation of the unfolded protein response, system A transporter 2 (SNAT2) transcription activity is repressed in HepG2 cells.
The Journal of biological chemistry. 283(41):27736-27747 [DOI] 10.1074/jbc.M803781200. [PMID] 18697751.
Mass spectrometric quantification of asparagine synthetase in circulating leukemia cells from acute lymphoblastic leukemia patients.
Journal of proteomics. 71(1):61-70 [DOI] 10.1016/j.jprot.2007.11.009. [PMID] 18541474.
MEK signaling is required for phosphorylation of eIF2alpha following amino acid limitation of HepG2 human hepatoma cells.
The Journal of biological chemistry. 283(16):10848-57 [DOI] 10.1074/jbc.M708320200. [PMID] 18287093.
Metabolic regulation of manganese superoxide dismutase expression via essential amino acid deprivation.
The Journal of biological chemistry. 283(16):10252-63 [DOI] 10.1074/jbc.M709944200. [PMID] 18187411.
Activation of the ATF3 gene through a co-ordinated amino acid-sensing response programme that controls transcriptional regulation of responsive genes following amino acid limitation.
The Biochemical journal. 401(1):299-307 [PMID] 16989641.
Alignment of the transcription start site coincides with increased transcriptional activity from the human asparagine synthetase gene following amino acid deprivation of HepG2 cells.
The Journal of nutrition. 136(10):2463-7 [PMID] 16988110.
An inhibitor of human asparagine synthetase suppresses proliferation of an L-asparaginase-resistant leukemia cell line.
Chemistry & biology. 13(12):1339-47 [PMID] 17185229.
Asparagine synthetase chemotherapy.
Annual review of biochemistry. 75:629-54 [PMID] 16756505.
Characterization of the amino acid response element within the human sodium-coupled neutral amino acid transporter 2 (SNAT2) System A transporter gene.
The Biochemical journal. 395(3):517-27 [PMID] 16445384.
Amino-acid limitation induces transcription from the human C/EBPbeta gene via an enhancer activity located downstream of the protein coding sequence.
The Biochemical journal. 391(Pt 3):649-58 [PMID] 16026328.
Interaction of RNA-binding proteins HuR and AUF1 with the human ATF3 mRNA 3′-untranslated region regulates its amino acid limitation-induced stabilization.
The Journal of biological chemistry. 280(41):34609-16 [PMID] 16109718.
Nutritional control of gene expression: how mammalian cells respond to amino acid limitation.
Annual review of nutrition. 25:59-85 [PMID] 16011459.
Amino acid deprivation induces the transcription rate of the human asparagine synthetase gene through a timed program of expression and promoter binding of nutrient-responsive basic region/leucine zipper transcription factors as well as localized histone acetylation.
The Journal of biological chemistry. 279(49):50829-39 [PMID] 15385533.
Human CCAAT/enhancer-binding protein beta gene expression is activated by endoplasmic reticulum stress through an unfolded protein response element downstream of the protein coding sequence.
The Journal of biological chemistry. 279(27):27948-56 [PMID] 15102854.
Induction of p21 and p27 expression by amino acid deprivation of HepG2 human hepatoma cells involves mRNA stabilization.
The Biochemical journal. 379(Pt 1):79-88 [PMID] 14715082.
Transcriptional control of the human sodium-coupled neutral amino acid transporter system A gene by amino acid availability is mediated by an intronic element.
The Journal of biological chemistry. 279(5):3463-71 [PMID] 14623874.
Amino acid deprivation and endoplasmic reticulum stress induce expression of multiple activating transcription factor-3 mRNA species that, when overexpressed in HepG2 cells, modulate transcription by the human asparagine synthetase promoter.
The Journal of biological chemistry. 278(40):38402-12 [PMID] 12881527.
Characterization of the nutrient-sensing response unit in the human asparagine synthetase promoter.
The Biochemical journal. 372(Pt 2):603-9 [PMID] 12628003.
Glucose-responsive expression of the human insulin promoter in HepG2 human hepatoma cells.
Annals of the New York Academy of Sciences. 1005:237-41 [PMID] 14679068.
Haem oxygenase 1 gene induction by glucose deprivation is mediated by reactive oxygen species via the mitochondrial electron-transport chain.
The Biochemical journal. 371(Pt 3):877-85 [PMID] 12585963.
Ontogeny of the neutral amino acid transporter SAT1/ATA1 in rat brain.
Brain research. Developmental brain research. 143(2):151-9 [PMID] 12855186.
Biosynthesis, intracellular targeting, and degradation of the EAAC1 glutamate/aspartate transporter in C6 glioma cells.
The Journal of biological chemistry. 277(41):38350-7 [PMID] 12151387.
Genomic sequences necessary for transcriptional activation by amino acid deprivation of mammalian cells.
The Journal of nutrition. 132(7):1801-4 [PMID] 12097650.
Glucose deprivation induces heme oxygenase-1 gene expression by a pathway independent of the unfolded protein response.
The Journal of biological chemistry. 277(3):1933-40 [PMID] 11707454.
Physiological importance of system A-mediated amino acid transport to rat fetal development.
American journal of physiology. Cell physiology. 282(1):C153-60 [PMID] 11742808.
Role of Sp1 and Sp3 in the nutrient-regulated expression of the human asparagine synthetase gene.
The Journal of biological chemistry. 277(19):16585-91 [PMID] 11867623.
Asparagine synthetase expression alone is sufficient to induce l-asparaginase resistance in MOLT-4 human leukaemia cells.
The Biochemical journal. 357(Pt 1):321-8 [PMID] 11415466.
CCAAT/enhancer-binding protein-beta is a mediator of the nutrient-sensing response pathway that activates the human asparagine synthetase gene.
The Journal of biological chemistry. 276(51):48100-7 [PMID] 11677247.
Multiple adaptive mechanisms affect asparagine synthetase substrate availability in asparaginase-resistant MOLT-4 human leukaemia cells.
The Biochemical journal. 358(Pt 1):59-67 [PMID] 11485552.
Activation of the human asparagine synthetase gene by the amino acid response and the endoplasmic reticulum stress response pathways occurs by common genomic elements.
The Journal of biological chemistry. 275(35):26976-85 [PMID] 10856289.
Activation of the unfolded protein response pathway induces human asparagine synthetase gene expression.
The Journal of biological chemistry. 274(44):31139-44 [PMID] 10531303.
Placental anionic and cationic amino acid transporter expression in growth hormone overexpressing and null IGF-II or null IGF-I receptor mice.
Placenta. 20(8):639-50 [PMID] 10527818.
Transcriptional regulation of the human asparagine synthetase gene by carbohydrate availability.
The Biochemical journal. 339 ( Pt 1)(Pt 1):151-8 [PMID] 10085239.
Activity and protein localization of multiple glutamate transporters in gestation day 14 vs. day 20 rat placenta.
The American journal of physiology. 274(3):C603-14 [DOI] 10.1152/ajpcell.1998.274.3.C603. [PMID] 9530091.
Response of placental amino acid transport to gestational age and intrauterine growth retardation.
The Proceedings of the Nutrition Society. 57(2):257-63 [PMID] 9656329.
A caveolar complex between the cationic amino acid transporter 1 and endothelial nitric-oxide synthase may explain the “arginine paradox”.
The Journal of biological chemistry. 272(50):31213-6 [PMID] 9395443.
Adaptive regulation of the cationic amino acid transporter-1 (Cat-1) in Fao cells.
The Journal of biological chemistry. 272(32):19951-7 [PMID] 9242663.
Amino acid control of asparagine synthetase: relation to asparaginase resistance in human leukemia cells.
The American journal of physiology. 272(5 Pt 1):C1691-9 [PMID] 9176161.
An expression system for mammalian amino acid transporters using a stably maintained episomal vector.
Analytical biochemistry. 254(2):208-14 [PMID] 9417778.
Demonstration of system y+L activity on the basal plasma membrane surface of rat placenta and developmentally regulated expression of 4F2HC mRNA.
Placenta. 18(8):643-8 [PMID] 9364599.
Identification of a system N-like Na(+)-dependent glutamine transport activity in rat brain neurons.
Journal of neurochemistry. 68(3):954-60 [PMID] 9048740.
Inhibition of endothelial cell amino acid transport System y+ by arginine analogs that inhibit nitric oxide synthase.
Biochimica et biophysica acta. 1324(1):133-41 [PMID] 9059506.
An example of nutrient control of gene expression: amino acid-dependent regulation of asparagine synthetase.
Clinical nutrition (Edinburgh, Scotland). 15(6):327-31 [PMID] 16844066.
Association of hepatic system A amino acid transporter with the membrane-cytoskeletal proteins ankyrin and fodrin.
Biochimica et biophysica acta. 1282(1):107-14 [PMID] 8679646.
Characterization and distribution of the neuronal glutamate transporter EAAC1 in rat brain.
The American journal of physiology. 270(1 Pt 1):C67-75 [PMID] 8772431.
Effect of low-protein diet-induced intrauterine growth retardation on rat placental amino acid transport.
The American journal of physiology. 271(1 Pt 1):C295-303 [PMID] 8760058.
Eukaryotic gene expression: metabolite control by amino acids.
Progress in nucleic acid research and molecular biology. 53:219-48 [PMID] 8650304.
Expressed human hippocampal ASCT1 amino acid transporter exhibits a pH-dependent change in substrate specificity.
Biochimica et biophysica acta. 1279(2):131-6 [PMID] 8603078.
Molecular biology of mammalian amino acid transporters.
Annual review of biochemistry. 65:305-36 [PMID] 8811182.
Rat placental amino acid transport after protein-deprivation-induced intrauterine growth retardation.
Biochemical Society transactions. 24(3):839-43 [PMID] 8878858.
Hepatic amino acid transport primary to the urea cycle in regulation of biologic neutrality.
Nutrition reviews. 53(3):74-6 [PMID] 7770188.
Amino acid-regulated gene expression in eukaryotic cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 8(1):13-9 [PMID] 8299885.
Cloning of rat asparagine synthetase and specificity of the amino acid-dependent control of its mRNA content.
The Biochemical journal. 304 ( Pt 3)(Pt 3):745-50 [PMID] 7818476.
Nuclear retention of the induced mRNA following amino acid-dependent transcriptional regulation of mammalian ribosomal proteins L17 and S25.
The Journal of biological chemistry. 269(13):9693-7 [PMID] 8144559.
Ontogeny and plasma-membrane domain localization of amino acid transport system L in rat liver.
The Biochemical journal. 301 ( Pt 3)(Pt 3):671-4 [PMID] 8053892.
Ontogeny of cationic amino acid transport systems in rat placenta.
The American journal of physiology. 267(3 Pt 1):C804-11 [PMID] 7943209.
Plasma membrane clustering of system y+ (CAT-1) amino acid transporter as detected by immunohistochemistry.
The American journal of physiology. 266(5 Pt 1):E817-24 [PMID] 8203520.
Protein modification of glutamine transporters in SV40-transformed hepatocytes and immunodetection of proteins associated with hepatic system N transport activity.
The Journal of nutrition. 124(8 Suppl):1493S-1498S [DOI] 10.1093/jn/124.suppl_8.1493S. [PMID] 8064408.
Cloning and expression of a novel Na(+)-dependent neutral amino acid transporter structurally related to mammalian Na+/glutamate cotransporters.
The Journal of biological chemistry. 268(21):15351-5 [PMID] 8340364.
Enhanced mRNA content in response to amino acid starvation for a 73 kDa protein of the inner mitochondrial membrane.
Biochemical and biophysical research communications. 193(3):1068-75 [PMID] 8323532.
Glucocorticoid regulation of splanchnic glutamine, alanine, glutamate, ammonia, and glutathione fluxes.
The American journal of physiology. 264(4 Pt 1):E526-33 [PMID] 8097375.
Recent advances in mammalian amino acid transport.
Annual review of nutrition. 13:137-65 [PMID] 8369142.
Identification of the protein responsible for hepatic system N amino acid transport activity.
The Journal of biological chemistry. 267(4):2370-4 [PMID] 1733938.
Plasma membrane domain localization and transcytosis of the glucagon-induced hepatic system A carrier.
The American journal of physiology. 263(6):E1021-8 [DOI] 10.1152/ajpendo.2006.263.6.E1021. [PMID] 1335691.
Amino acid-dependent increase in hepatic system N activity is linked to cell swelling.
The Journal of biological chemistry. 266(12):7376-81 [PMID] 2019573.
Functional reconstitution of the hepatic system N amino acid transport activity.
The Biochemical journal. 274 ( Pt 1)(Pt 1):97-101 [PMID] 2001257.
Glutamine transport by basolateral plasma-membrane vesicles prepared from rabbit intestine.
The Biochemical journal. 277 ( Pt 3)(Pt 3):687-91 [PMID] 1908221.
Identification of an amino acid-regulated mRNA from rat liver as the mammalian equivalent of bacterial ribosomal protein L22.
The Journal of biological chemistry. 266(26):16969-72 [PMID] 1894596.
Characteristics and regulation of hepatic glutamine transport.
JPEN. Journal of parenteral and enteral nutrition. 14(4 Suppl):51S-55S [PMID] 2402055.
Expression of the mammalian system A neutral amino acid transporter in Xenopus oocytes.
The Journal of biological chemistry. 265(23):13914-7 [PMID] 2380194.
Hormone-induced system A amino acid transport activity in rat liver plasma membrane and Golgi vesicles. Evidence for a differential sensitivity to inactivation by N-ethylmaleimide during carrier maturation.
The Journal of biological chemistry. 265(3):1470-5 [PMID] 2295641.
Molecular cloning of an amino acid-regulated mRNA (amino acid starvation-induced) in rat hepatoma cells.
The Journal of biological chemistry. 265(29):17844-8 [PMID] 2211664.
Sodium-dependent neutral amino acid transport by human liver plasma membrane vesicles.
The Journal of biological chemistry. 265(24):14321-6 [PMID] 2387853.
Substrate-dependent adaptive regulation and trans-inhibition of System A-mediated amino acid transport. Studies using rat hepatoma plasma membrane vesicles.
Biochimica et biophysica acta. 1022(3):325-32 [PMID] 1690572.
Characterization of neutral and cationic amino acid transport in Xenopus oocytes.
Journal of cellular physiology. 141(3):645-52 [PMID] 2592432.
Measurement of amino acid transport by hepatocytes in suspension or monolayer culture.
Methods in enzymology. 173:564-75 [PMID] 2550731.
Solubilization and reconstitution characteristics of hepatic system A-mediated amino acid transport.
The Journal of biological chemistry. 264(9):4805-11 [PMID] 2925669.
Growth-dependent regulation of system A in SV40-transformed fetal rat hepatocytes.
The American journal of physiology. 255(3 Pt 1):C261-70 [PMID] 2844092.
Inactivation of amino acid transport in rat hepatocytes and hepatoma cells by PCMBS.
The American journal of physiology. 255(3 Pt 1):C340-5 [PMID] 2844094.
Transport of branched-chain amino acids and their corresponding 2-keto acids by mammalian cells.
Methods in enzymology. 166:252-60 [PMID] 3071709.
Evidence for inherent differences in the system A carrier from normal and transformed liver tissue. Differential inactivation and substrate protection in membrane vesicles and reconstituted proteoliposomes.
The Journal of biological chemistry. 262(26):12565-9 [PMID] 3040757.
Production of monospecific antibodies to a low-abundance hepatic membrane protein using nitrocellulose immobilized protein as antigen.
Analytical biochemistry. 163(1):136-42 [PMID] 3304007.
Solubilization and reconstitution of hepatic System A-mediated amino acid transport. Preparation of proteoliposomes containing glucagon-stimulated transport activity.
Biochimica et biophysica acta. 899(1):51-8 [PMID] 3567191.
Cis-inhibition, trans-inhibition, and repression of hepatic amino acid transport mediated by System A. Substrate specificity and other properties.
The Journal of biological chemistry. 261(4):1514-20 [PMID] 3944097.
Maintenance of glucagon-stimulated system A amino acid transport activity in rat liver plasma membrane vesicles.
Biochimica et biophysica acta. 856(3):428-36 [PMID] 3964688.
Substrate regulation of hepatic system A transport activity after induction by substrate starvation or glucagon.
Federation proceedings. 45(10):2438-41 [PMID] 3743783.
System A transport activity in normal rat hepatocytes and transformed liver cells: substrate protection from inactivation by sulfhydryl-modifying reagents.
Journal of cellular physiology. 129(3):321-8 [PMID] 3023402.
Adaptive regulation of neutral amino acid transport System A in rat H4 hepatoma cells.
Journal of cellular physiology. 122(2):290-8 [PMID] 2578476.
Amino acid-dependent inactivation of glucagon-induced System A transport activity in cultured rat hepatocytes.
Molecular and cellular endocrinology. 43(1):61-9 [PMID] 4065425.
Characteristics and hormonal regulation of amino acid transport system A in isolated rat hepatocytes.
Current topics in cellular regulation. 25:133-63 [PMID] 2410197.
Preferential binding of vasoactive intestinal peptide to hepatic nonparenchymal cells.
The American journal of physiology. 248(6 Pt 1):G663-9 [PMID] 2988343.
Amino acid activation of amino acid transport System N early in primary cultures of rat hepatocytes.
Journal of cellular physiology. 121(1):133-8 [PMID] 6480708.
Amino acid transport systems.
Nature. 311(5984) [PMID] 6482960.
Induction and decay of amino acid transport in the liver. Turnover of transport activity in isolated hepatocytes after stimulation by diabetes or glucagon.
The Journal of biological chemistry. 259(6):3519-25 [PMID] 6200477.
Induction of amino acid transport system A in rat hepatocytes is blocked by tunicamycin.
The Journal of biological chemistry. 258(19):11851-5 [PMID] 6352704.
Plasma membrane transport of 2-ketoisocaproate by rat hepatocytes in primary culture.
The Journal of biological chemistry. 258(19):11524-7 [PMID] 6352700.
Regulation of neutral amino acid transport in hepatocytes isolated from adrenalectomized rats.
Journal of cellular physiology. 114(1):45-52 [PMID] 6338022.
The regulation of neutral amino acid transport in mammalian cells.
Biochimica et biophysica acta. 737(2):267-84 [PMID] 6303424.
Amino acid transport in isolated rat hepatocytes.
The Journal of membrane biology. 69(1):1-12 [PMID] 6811749.
Evidence for two Na+-independent neutral amino acid transport systems in primary cultures of rat hepatocytes. Time-dependent changes in activity.
The Journal of biological chemistry. 257(20):12006-11 [PMID] 7118928.
Heterogeneity of Na+-independent 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid and L-leucine transport in isolated rat hepatocytes in primary culture.
Biochemical and biophysical research communications. 104(1):307-13 [PMID] 7073676.
Incomplete correspondence between repressive and substrate action by amino acids on transport systems A and N in monolayered rat hepatocytes.
The Journal of biological chemistry. 257(1):345-8 [PMID] 7053375.
Neutral amino acid transport in hepatocytes isolated from streptozotocin-induced diabetic rats.
The Journal of biological chemistry. 257(24):14960-7 [PMID] 7174677.
Transport system a is not responsive to hormonal stimulation in primary cultures of fetal rat hepatocytes.
Biochemical and biophysical research communications. 108(3):1113-9 [PMID] 6758770.
Characteristics of system ASC for transport of neutral amino acids in the isolated rat hepatocyte.
The Journal of biological chemistry. 256(7):3304-12 [PMID] 7204404.
Neutral amino acid transport systems in Chinese hamster ovary cells.
The Journal of biological chemistry. 256(11):5422-7 [PMID] 7240147.
Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine, and closely related analogs.
The Journal of biological chemistry. 255(9):4011-9 [PMID] 7372663.
Energization of amino acid transport in energy-depleted Ehrlich cells and plasma membrane vesicles.
Biochimica et biophysica acta. 599(1):175-90 [PMID] 7397146.
The relation between membrane potential and the transport activity of system A and L in plasma membrane vesicles of the Ehrlich cell.
Membrane biochemistry. 3(1-2):155-68 [PMID] 7432185.
Cysteine as a system-specific substrate for transport system ASC in rat hepatocytes.
Biochemical and biophysical research communications. 88(2):744-51 [PMID] 465067.
Electron-transferring enzymes in the plasma membrane of the Ehrlich ascites tumor cell.
Biochemistry. 18(8):1525-30 [PMID] 427130.
Hormonal control of amino acid transport in the liver of rats exposed to whole-body gamma irradiation.
Radiation research. 73(2):360-72 [PMID] 204956.
Hormonal regulation of hepatic amino acid transport.
Journal of supramolecular structure. 6(2):191-204 [PMID] 198613.
The role of stimulated amino acid transport in promoting glycogenesis in the irradiated rat.
Radiation research. 66(3):597-608 [PMID] 935346.
Accumulation of alpha-aminoisobutyric acid by rat tissues after whole-body gamma-irradiation.
Radiation research. 64(3):546-54 [PMID] 1197657.
Mar 2020 – Feb 2023
Asparagine Synthetase Deficiency
Role: Principal Investigator
Funding: NATL INST OF HLTH NICHD
Aug 2015 – Jul 2021
Nutritional Control of Cancer Cell Function by Amino Acids
Role: Principal Investigator
Funding: NATL INST OF HLTH NCI
Aug 2011 – Jul 2016
Amino Acid Regulation of Alternative Splicing
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDDK
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