Philip J Laipis,
Professor Emeritus
Department:
MD-BIOCHEM / MOLECULAR BIOL
Business Email:
plaipis@ufl.edu
About Philip J Laipis
Professor Philip Laipis received the Ph.D. in genetics from Stanford University in 1972. His thesis research with Dr. A.T. Ganesan examined the role of DNA polymerases and DNA ligase in repair and recombination of B. subtilis DNA. He joined A.J. Levine’s laboratory at Princeton University as an NIH postdoctoral fellow, and he studied replicating SV40 viral DNA. He joined the University of Florida in 1974. He rose to the rank of Professor in 1986. Dr. Laipis has served on the NIH Physiological Chemistry and Therapeutic Approaches to Genetic Disease Study Sections and since 1997 has been the Associate Chair of the Department.
Teaching Profile
Courses Taught
2011-2013
BCH4905 Biochemistry Senior Research
2009,2011-2013
BCH4024 Introduction to Biochemistry and Molecular Biology
2011-2013
BCH6040 Research Discussion in Biochemistry and Molecular Biology
2007-2012
BCH5413 Mammalian Molecular Biology and Genetics
2010,2012
BCH6415 Advanced Molecular and Cell Biology
2008-2011
GMS5905 Special Topics in Biomedical Sciences
Research Profile
Professor Philip Laipis received the Ph.D. in genetics from Stanford University in 1972. His thesis research with Dr. A.T. Ganesan examined the role of DNA polymerases and DNA ligase in repair and recombination of B. subtilis DNA. He joined A.J. Levine’s laboratory at Princeton University as an NIH postdoctoral fellow, and he studied replicating SV40 viral DNA. He joined the University of Florida in 1974. He rose to the rank of Professor in 1986. Dr. Laipis has served on the NIH Physiological Chemistry and Therapeutic Approaches to Genetic Disease Study Sections and since 1997 has been the Associate Chair of the Department.
Publications
Academic Articles
2018
Maternal phenylketonuria syndrome: studies in mice suggest a potential approach to a continuing problem.
Pediatric research.
83(4):889-896
[DOI] 10.1038/pr.2017.323.
[PMID] 29278642.
2017
Partial rescue of neuropathology in the murine model of PKU following administration of recombinant phenylalanine ammonia lyase (pegvaliase).
Molecular genetics and metabolism.
122(1-2):33-35
[DOI] 10.1016/j.ymgme.2017.04.013.
[PMID] 28506393.
2007
Epilepsy in phenylketonuria: a complex dependence on serum phenylalanine levels.
Epilepsia.
48(6):1143-50
[PMID] 17484755.
2007
PKU is a reversible neurodegenerative process within the nigrostriatum that begins as early as 4 weeks of age in Pah(enu2) mice.
Brain research.
1127(1):136-50
[PMID] 17112485.
2007
Structural and kinetic analysis of proton shuttle residues in the active site of human carbonic anhydrase III.
Proteins.
68(1):337-43
[PMID] 17427958.
2006
A murine model for human sepiapterin-reductase deficiency.
American journal of human genetics.
78(4):575-87
[PMID] 16532389.
2005
Human carbonic anhydrase III: structural and kinetic study of catalysis and proton transfer.
Biochemistry.
44(30):10046-53
[PMID] 16042381.
2005
Impaired glutamatergic synaptic transmission in the PKU brain.
Molecular genetics and metabolism.
86 Suppl 1:S34-42
[PMID] 16153867.
2005
Long-term changes in glutamatergic synaptic transmission in phenylketonuria.
Brain : a journal of neurology.
128(Pt 2):300-7
[PMID] 15634735.
2005
Pathologic and immunohistochemical findings in hypothalamic and mesencephalic regions in the pah(enu2) mouse model for phenylketonuria.
Pediatric research.
58(2):283-7
[PMID] 16055931.
2004
Activation of carbonic anhydrase II by active-site incorporation of histidine analogs.
Archives of biochemistry and biophysics.
421(2):283-9
[PMID] 14984209.
2004
Glucose deprivation enhances targeting of GLUT1 to lipid rafts in 3T3-L1 adipocytes.
American journal of physiology. Endocrinology and metabolism.
286(4):E568-76
[PMID] 14665446.
2004
Locus control region elements HS2 and HS3 in combination with chromatin boundaries confer high-level expression of a human beta-globin transgene in a centromeric region.
Genes to cells : devoted to molecular & cellular mechanisms.
9(11):1043-53
[PMID] 15507116.
2003
Direct atmospheric pressure coupling of polyacrylamide gel electrophoresis to mass spectrometry for rapid protein sequence analysis.
Journal of proteome research.
2(6):610-7
[PMID] 14692454.
2003
Using evolutionary rates to investigate protein functional divergence and conservation. A case study of the carbonic anhydrases.
Genetics.
164(4):1261-9
[PMID] 12930737.
2002
Chemical rescue in catalysis by human carbonic anhydrases II and III.
Biochemistry.
41(9):3235-42
[PMID] 11863462.
2002
Glycogen phosphorylase is activated in response to glucose deprivation but is not responsible for enhanced glucose transport activity in 3T3-L1 adipocytes.
Biochimica et biophysica acta.
1570(1):53-62
[PMID] 11960689.
2002
Kinetic analysis of multiple proton shuttles in the active site of human carbonic anhydrase.
The Journal of biological chemistry.
277(41):38870-6
[PMID] 12171926.
2002
Laser desorption-atmospheric pressure chemical ionization: a novel ion source for the direct coupling of polyacrylamide gel electrophoresis to mass spectrometry.
Journal of mass spectrometry : JMS.
37(11):1163-7
[PMID] 12447894.
2002
Proton transfer within the active-site cavity of carbonic anhydrase III.
Biochimica et biophysica acta.
1599(1-2):21-7
[PMID] 12484342.
2001
Effect of DNA-dependent protein kinase on the molecular fate of the rAAV2 genome in skeletal muscle.
Proceedings of the National Academy of Sciences of the United States of America.
98(7):4084-8
[PMID] 11274433.
2001
Stable therapeutic serum levels of human alpha-1 antitrypsin (AAT) after portal vein injection of recombinant adeno-associated virus (rAAV) vectors.
Gene therapy.
8(17):1299-306
[PMID] 11571566.
2001
Structural and kinetic analysis of the chemical rescue of the proton transfer function of carbonic anhydrase II.
Biochemistry.
40(6):1741-8
[PMID] 11327835.
2001
The catalytic properties of human carbonic anhydrase IX.
Biochemical and biophysical research communications.
288(3):666-9
[PMID] 11676494.
2001
Use of recombinant angiostatin to prevent retinal neovascularization.
Transactions of the American Clinical and Climatological Association.
112:68-75; discussion 75
[PMID] 11413784.
1999
Carbon dioxide transport by proteic and facilitated transport membranes.
Life support & biosphere science : international journal of earth space.
6(4):293-302
[PMID] 11543269.
1999
Introduction of histidine analogs leads to enhanced proton transfer in carbonic anhydrase V.
Archives of biochemistry and biophysics.
361(2):264-70
[PMID] 9882455.
1999
Proton transfer to residues of basic pK(a) during catalysis by carbonic anhydrase.
Biochimica et biophysica acta.
1434(1):1-5
[PMID] 10556554.
1998
Intramolecular proton transfer from multiple sites in catalysis by murine carbonic anhydrase V.
Biochemistry.
37(20):7649-55
[PMID] 9585580.
1998
Isolation and expression of murine carbonic anhydrase IV.
Protein expression and purification.
12(1):7-16
[PMID] 9473451.
1998
Properties of intramolecular proton transfer in carbonic anhydrase III.
Biophysical journal.
74(6):3182-9
[PMID] 9635771.
1998
Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination.
Protein science : a publication of the Protein Society.
7(3):556-63
[PMID] 9541386.
1998
The catalytic properties of murine carbonic anhydrase VII.
Biochemistry.
37(30):10837-45
[PMID] 9692974.
1997
Catalytic properties of murine carbonic anhydrase IV.
The Journal of biological chemistry.
272(21):13512-8
[PMID] 9153196.
1997
Glutamate and aspartate as proton shuttles in mutants of carbonic anhydrase.
Biochemistry.
36(50):15758-64
[PMID] 9398305.
1996
Construction of heteroplasmic mice containing two mitochondrial DNA genotypes by micromanipulation of single-cell embryos.
Methods in enzymology.
264:345-57
[PMID] 8965708.
1996
Structure-based design of an intramolecular proton transfer site in murine carbonic anhydrase V.
Biochemistry.
35(36):11605-11
[PMID] 8794740.
1995
Multiple deletions are detectable in mitochondrial DNA of aging mice.
The Journal of biological chemistry.
270(42):24769-75
[PMID] 7559594.
1995
Proton transfer by histidine 67 in site-directed mutants of human carbonic anhydrase III.
Biochemistry.
34(26):8492-8
[PMID] 7599138.
1995
Structure determination of murine mitochondrial carbonic anhydrase V at 2.45-A resolution: implications for catalytic proton transfer and inhibitor design.
Proceedings of the National Academy of Sciences of the United States of America.
92(24):10949-53
[PMID] 7479916.
1994
Analysis of expressed sequence tags from Plasmodium falciparum.
Molecular and biochemical parasitology.
66(1):97-104
[PMID] 7984191.
1994
Catalytic properties of mouse carbonic anhydrase V.
The Journal of biological chemistry.
269(40):24742-6
[PMID] 7929150.
1994
Interactions of active-site residues and catalytic activity of human carbonic anhydrase III.
The Journal of biological chemistry.
269(37):23002-6
[PMID] 8083199.
1993
Influence of amino acid replacement at position 198 on catalytic properties of zinc-bound water in human carbonic anhydrase III.
Biochemistry.
32(22):5786-91
[PMID] 8504098.
1993
Interaction and influence of phenylalanine-198 and threonine-199 on catalysis by human carbonic anhydrase III.
Biochemistry.
32(31):7861-5
[PMID] 8347590.
1993
Multiple copies of the bone-specific osteocalcin gene in mouse and rat.
Endocrinology.
133(6):3050-3
[PMID] 8243336.
1993
Rate-equilibria relationships in intramolecular proton transfer in human carbonic anhydrase III.
Biochemistry.
32(40):10757-62
[PMID] 8399223.
1993
Transcribed heteroplasmic repeated sequences in the porcine mitochondrial DNA D-loop region.
Journal of molecular evolution.
37(1):36-7
[PMID] 8360917.
1992
A T7 expression vector optimized for site-directed mutagenesis using oligodeoxyribonucleotide cassettes.
Gene.
117(1):113-7
[PMID] 1644301.
1992
Mispairing and compensational changes during the evolution of mitochondrial ribosomal RNA.
Molecular biology and evolution.
9(4):770-4
[PMID] 1378523.
1991
Catalytic enhancement of human carbonic anhydrase III by replacement of phenylalanine-198 with leucine.
Biochemistry.
30(34):8463-70
[PMID] 1909176.
1991
Enhancement of the catalytic properties of human carbonic anhydrase III by site-directed mutagenesis.
Biochemistry.
30(6):1484-90
[PMID] 1899618.
1991
Identification of an amino acid-regulated mRNA from rat liver as the mammalian equivalent of bacterial ribosomal protein L22.
The Journal of biological chemistry.
266(26):16969-72
[PMID] 1894596.
1990
Buffer enhancement of proton transfer in catalysis by human carbonic anhydrase III.
Biochemistry.
29(27):6400-5
[PMID] 2169869.
1990
Enhancement of the catalytic activity of carbonic anhydrase III by phosphates.
The Journal of biological chemistry.
265(36):22270-4
[PMID] 2125046.
1989
Rapid segregation of heteroplasmic bovine mitochondria.
Nucleic acids research.
17(18):7325-31
[PMID] 2798094.
1988
Oral squamous papillomas: detection of HPV DNA by in situ hybridization.
Oral surgery, oral medicine, and oral pathology.
65(5):545-50
[PMID] 2836773.
1988
Unequal partitioning of bovine mitochondrial genotypes among siblings.
Proceedings of the National Academy of Sciences of the United States of America.
85(21):8107-10
[PMID] 2460862.
1987
Demonstration of human papillomavirus DNA in oral condyloma acuminatum.
Journal of oral pathology.
16(5):266-72
[PMID] 2821213.
1987
Human papillomavirus type 2 DNA in oral and labial verruca vulgaris.
Journal of cutaneous pathology.
14(6):319-25
[PMID] 2832456.
1986
Anatomical, cellular and molecular analysis of 8,000-yr-old human brain tissue from the Windover archaeological site.
Nature.
323(6091):803-6
[PMID] 2430186.
1986
Conserved restriction sites within the ribosomal RNA genes of vertebrates.
Biochimica et biophysica acta.
866(1):19-25
[PMID] 3004584.
1986
Template-directed arrest of mammalian mitochondrial DNA synthesis.
Molecular and cellular biology.
6(4):1261-7
[PMID] 3785163.
1984
Heterogeneous mitochondrial DNA D-loop sequences in bovine tissue.
Cell.
37(3):1001-7
[PMID] 6744410.
1983
Nucleotide sequence evidence for rapid genotypic shifts in the bovine mitochondrial DNA D-loop.
Nature.
306(5941):400-2
[PMID] 6646218.
1982
Mitochondrial DNA copy number in bovine oocytes and somatic cells.
Developmental biology.
94(1):246-51
[PMID] 6295849.
1982
Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows.
Proceedings of the National Academy of Sciences of the United States of America.
79(15):4686-90
[PMID] 6289312.
1980
Genetic mapping of bovine mitochondrial DNA from a single animal.
Gene.
8(2):193-209
[PMID] 7358273.
1979
A physical map of bovine mitochondrial DNA from a single animal.
Biochimica et biophysica acta.
565(1):22-32
[PMID] 508763.
1973
DNA replication in SV40-infected cells. IX. The inhibition of a gap-filling step during discontinuous synthesis of SV40 DNA.
Virology.
56(2):580-94
[PMID] 4357056.
Contact Details
Emails:
- Business:
- plaipis@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100245
GAINESVILLE FL 32610 - Business Street:
-
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIO
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIO
COLLEGE OF MEDICINE
GAINESVILLE FL 326100001