Philip J Laipis,
PROF & ASO CHR
Department:
MD-BIOCHEM / MOLECULAR BIOL
Business Email:
plaipis@ufl.edu
Teaching Profile
Courses Taught
2011-2013
BCH4905 Biochemistry Senior Research
2009,2011-2013
BCH4024 Introduction to Biochemistry and Molecular Biology
2011-2013
BCH6040 Research Discussion in Biochemistry and Molecular Biology
2007-2012
BCH5413 Mammalian Molecular Biology and Genetics
2010,2012
BCH6415 Advanced Molecular and Cell Biology
2008-2011
GMS5905 Special Topics in Biomedical Sciences
Publications
2018
Maternal phenylketonuria syndrome: studies in mice suggest a potential approach to a continuing problem.
Pediatric research.
83(4):889-896
[DOI] 10.1038/pr.2017.323.
[PMID] 29278642.
2017
Partial rescue of neuropathology in the murine model of PKU following administration of recombinant phenylalanine ammonia lyase (pegvaliase).
Molecular genetics and metabolism.
122(1-2):33-35
[DOI] 10.1016/j.ymgme.2017.04.013.
[PMID] 28506393.
2007
Epilepsy in phenylketonuria: a complex dependence on serum phenylalanine levels.
Epilepsia.
48(6):1143-50
[PMID] 17484755.
2007
PKU is a reversible neurodegenerative process within the nigrostriatum that begins as early as 4 weeks of age in Pah(enu2) mice.
Brain research.
1127(1):136-50
[PMID] 17112485.
2007
Structural and kinetic analysis of proton shuttle residues in the active site of human carbonic anhydrase III.
Proteins.
68(1):337-43
[PMID] 17427958.
2006
A murine model for human sepiapterin-reductase deficiency.
American journal of human genetics.
78(4):575-87
[PMID] 16532389.
2005
Human carbonic anhydrase III: structural and kinetic study of catalysis and proton transfer.
Biochemistry.
44(30):10046-53
[PMID] 16042381.
2005
Impaired glutamatergic synaptic transmission in the PKU brain.
Molecular genetics and metabolism.
86 Suppl 1:S34-42
[PMID] 16153867.
2005
Long-term changes in glutamatergic synaptic transmission in phenylketonuria.
Brain : a journal of neurology.
128(Pt 2):300-7
[PMID] 15634735.
2005
Pathologic and immunohistochemical findings in hypothalamic and mesencephalic regions in the pah(enu2) mouse model for phenylketonuria.
Pediatric research.
58(2):283-7
[PMID] 16055931.
2004
Activation of carbonic anhydrase II by active-site incorporation of histidine analogs.
Archives of biochemistry and biophysics.
421(2):283-9
[PMID] 14984209.
2004
Glucose deprivation enhances targeting of GLUT1 to lipid rafts in 3T3-L1 adipocytes.
American journal of physiology. Endocrinology and metabolism.
286(4):E568-76
[PMID] 14665446.
2004
Locus control region elements HS2 and HS3 in combination with chromatin boundaries confer high-level expression of a human beta-globin transgene in a centromeric region.
Genes to cells : devoted to molecular & cellular mechanisms.
9(11):1043-53
[PMID] 15507116.
2003
Direct atmospheric pressure coupling of polyacrylamide gel electrophoresis to mass spectrometry for rapid protein sequence analysis.
Journal of proteome research.
2(6):610-7
[PMID] 14692454.
2003
Using evolutionary rates to investigate protein functional divergence and conservation. A case study of the carbonic anhydrases.
Genetics.
164(4):1261-9
[PMID] 12930737.
2002
Chemical rescue in catalysis by human carbonic anhydrases II and III.
Biochemistry.
41(9):3235-42
[PMID] 11863462.
2002
Kinetic analysis of multiple proton shuttles in the active site of human carbonic anhydrase.
The Journal of biological chemistry.
277(41):38870-6
[PMID] 12171926.
2002
Proton transfer within the active-site cavity of carbonic anhydrase III.
Biochimica et biophysica acta.
1599(1-2):21-7
[PMID] 12484342.
2001
Effect of DNA-dependent protein kinase on the molecular fate of the rAAV2 genome in skeletal muscle.
Proceedings of the National Academy of Sciences of the United States of America.
98(7):4084-8
[PMID] 11274433.
2001
Structural and kinetic analysis of the chemical rescue of the proton transfer function of carbonic anhydrase II.
Biochemistry.
40(6):1741-8
[PMID] 11327835.
2001
Use of recombinant angiostatin to prevent retinal neovascularization.
Transactions of the American Clinical and Climatological Association.
112:68-75; discussion 75
[PMID] 11413784.
1999
Carbon dioxide transport by proteic and facilitated transport membranes.
Life support & biosphere science : international journal of earth space.
6(4):293-302
[PMID] 11543269.
1999
Introduction of histidine analogs leads to enhanced proton transfer in carbonic anhydrase V.
Archives of biochemistry and biophysics.
361(2):264-70
[PMID] 9882455.
1999
Proton transfer to residues of basic pK(a) during catalysis by carbonic anhydrase.
Biochimica et biophysica acta.
1434(1):1-5
[PMID] 10556554.
1998
Intramolecular proton transfer from multiple sites in catalysis by murine carbonic anhydrase V.
Biochemistry.
37(20):7649-55
[PMID] 9585580.
1998
Isolation and expression of murine carbonic anhydrase IV.
Protein expression and purification.
12(1):7-16
[PMID] 9473451.
1998
Properties of intramolecular proton transfer in carbonic anhydrase III.
Biophysical journal.
74(6):3182-9
[PMID] 9635771.
1998
Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination.
Protein science : a publication of the Protein Society.
7(3):556-63
[PMID] 9541386.
1998
The catalytic properties of murine carbonic anhydrase VII.
Biochemistry.
37(30):10837-45
[PMID] 9692974.
1997
Catalytic properties of murine carbonic anhydrase IV.
The Journal of biological chemistry.
272(21):13512-8
[PMID] 9153196.
1997
Glutamate and aspartate as proton shuttles in mutants of carbonic anhydrase.
Biochemistry.
36(50):15758-64
[PMID] 9398305.
1996
Construction of heteroplasmic mice containing two mitochondrial DNA genotypes by micromanipulation of single-cell embryos.
Methods in enzymology.
264:345-57
[PMID] 8965708.
1996
Structure-based design of an intramolecular proton transfer site in murine carbonic anhydrase V.
Biochemistry.
35(36):11605-11
[PMID] 8794740.
1995
Multiple deletions are detectable in mitochondrial DNA of aging mice.
The Journal of biological chemistry.
270(42):24769-75
[PMID] 7559594.
1995
Proton transfer by histidine 67 in site-directed mutants of human carbonic anhydrase III.
Biochemistry.
34(26):8492-8
[PMID] 7599138.
1995
Structure determination of murine mitochondrial carbonic anhydrase V at 2.45-A resolution: implications for catalytic proton transfer and inhibitor design.
Proceedings of the National Academy of Sciences of the United States of America.
92(24):10949-53
[PMID] 7479916.
1994
Analysis of expressed sequence tags from Plasmodium falciparum.
Molecular and biochemical parasitology.
66(1):97-104
[PMID] 7984191.
1994
Catalytic properties of mouse carbonic anhydrase V.
The Journal of biological chemistry.
269(40):24742-6
[PMID] 7929150.
1994
Interactions of active-site residues and catalytic activity of human carbonic anhydrase III.
The Journal of biological chemistry.
269(37):23002-6
[PMID] 8083199.
1993
Influence of amino acid replacement at position 198 on catalytic properties of zinc-bound water in human carbonic anhydrase III.
Biochemistry.
32(22):5786-91
[PMID] 8504098.
1993
Interaction and influence of phenylalanine-198 and threonine-199 on catalysis by human carbonic anhydrase III.
Biochemistry.
32(31):7861-5
[PMID] 8347590.
1993
Multiple copies of the bone-specific osteocalcin gene in mouse and rat.
Endocrinology.
133(6):3050-3
[PMID] 8243336.
1993
Rate-equilibria relationships in intramolecular proton transfer in human carbonic anhydrase III.
Biochemistry.
32(40):10757-62
[PMID] 8399223.
1993
Transcribed heteroplasmic repeated sequences in the porcine mitochondrial DNA D-loop region.
Journal of molecular evolution.
37(1):36-7
[PMID] 8360917.
1992
A T7 expression vector optimized for site-directed mutagenesis using oligodeoxyribonucleotide cassettes.
Gene.
117(1):113-7
[PMID] 1644301.
1992
Mispairing and compensational changes during the evolution of mitochondrial ribosomal RNA.
Molecular biology and evolution.
9(4):770-4
[PMID] 1378523.
1991
Catalytic enhancement of human carbonic anhydrase III by replacement of phenylalanine-198 with leucine.
Biochemistry.
30(34):8463-70
[PMID] 1909176.
1991
Enhancement of the catalytic properties of human carbonic anhydrase III by site-directed mutagenesis.
Biochemistry.
30(6):1484-90
[PMID] 1899618.
1991
Identification of an amino acid-regulated mRNA from rat liver as the mammalian equivalent of bacterial ribosomal protein L22.
The Journal of biological chemistry.
266(26):16969-72
[PMID] 1894596.
1990
Buffer enhancement of proton transfer in catalysis by human carbonic anhydrase III.
Biochemistry.
29(27):6400-5
[PMID] 2169869.
1990
Enhancement of the catalytic activity of carbonic anhydrase III by phosphates.
The Journal of biological chemistry.
265(36):22270-4
[PMID] 2125046.
1989
Rapid segregation of heteroplasmic bovine mitochondria.
Nucleic acids research.
17(18):7325-31
[PMID] 2798094.
1988
Oral squamous papillomas: detection of HPV DNA by in situ hybridization.
Oral surgery, oral medicine, and oral pathology.
65(5):545-50
[PMID] 2836773.
1988
Unequal partitioning of bovine mitochondrial genotypes among siblings.
Proceedings of the National Academy of Sciences of the United States of America.
85(21):8107-10
[PMID] 2460862.
1987
Demonstration of human papillomavirus DNA in oral condyloma acuminatum.
Journal of oral pathology.
16(5):266-72
[PMID] 2821213.
1987
Human papillomavirus type 2 DNA in oral and labial verruca vulgaris.
Journal of cutaneous pathology.
14(6):319-25
[PMID] 2832456.
1986
Anatomical, cellular and molecular analysis of 8,000-yr-old human brain tissue from the Windover archaeological site.
Nature.
323(6091):803-6
[PMID] 2430186.
1986
Conserved restriction sites within the ribosomal RNA genes of vertebrates.
Biochimica et biophysica acta.
866(1):19-25
[PMID] 3004584.
1986
Template-directed arrest of mammalian mitochondrial DNA synthesis.
Molecular and cellular biology.
6(4):1261-7
[PMID] 3785163.
1984
Heterogeneous mitochondrial DNA D-loop sequences in bovine tissue.
Cell.
37(3):1001-7
[PMID] 6744410.
1983
Nucleotide sequence evidence for rapid genotypic shifts in the bovine mitochondrial DNA D-loop.
Nature.
306(5941):400-2
[PMID] 6646218.
1982
Mitochondrial DNA copy number in bovine oocytes and somatic cells.
Developmental biology.
94(1):246-51
[PMID] 6295849.
1982
Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows.
Proceedings of the National Academy of Sciences of the United States of America.
79(15):4686-90
[PMID] 6289312.
1980
Genetic mapping of bovine mitochondrial DNA from a single animal.
Gene.
8(2):193-209
[PMID] 7358273.
1979
A physical map of bovine mitochondrial DNA from a single animal.
Biochimica et biophysica acta.
565(1):22-32
[PMID] 508763.
1973
DNA replication in SV40-infected cells. IX. The inhibition of a gap-filling step during discontinuous synthesis of SV40 DNA.
Virology.
56(2):580-94
[PMID] 4357056.
Contact Details
Emails:
- Business:
- plaipis@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100245
GAINESVILLE FL 32610 - Business Street:
-
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIO
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIO
COLLEGE OF MEDICINE
GAINESVILLE FL 326100001