Mario Mietzsch,
AST SCTST
Department:
MD-BIOCHEM / MOLECULAR BIOL
Business Phone:
(352) 846-1836
Business Email:
mario.mietzsch@ufl.edu
Teaching Profile
Courses Taught
2020-2022
GMS6251 Molecular Therapy I ? Vectors and Molecular Mechanisms
2021
BCH6747 Structural Biology/Advanced Physical Biochemistry: Spectroscopy and Hydrodynamics
2021-2024
BCH6740 Physical Biochemistry/Structural Biology
Research Profile
Open Researcher and Contributor ID (ORCID)
0000-0001-8957-3363
Publications
2024
Overcoming resolution attenuation during tilted cryo-EM data collection.
Nature communications.
15(1)
[DOI] 10.1038/s41467-023-44555-7.
[PMID] 38195598.
2024
Single-Particle Cryo-EM of AAV2 at Various Tilts
EMPIAR dataset.
[DOI] 10.6019/empiar-11791.
2024
Single-Particle Cryo-EM of RNA Polymerase at Various Tilts
EMPIAR dataset.
[DOI] 10.6019/empiar-11797.
2023
Development of new adeno-associated virus capsid variants for targeted gene delivery to human cardiomyocytes.
Molecular therapy. Methods & clinical development.
30:459-473
[DOI] 10.1016/j.omtm.2023.08.010.
[PMID] 37674904.
2023
Editorial: Parvoviruses: from basic research to biomedical and biotechnological applications.
Frontiers in microbiology.
14
[DOI] 10.3389/fmicb.2023.1194926.
[PMID] 37256056.
2023
Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2023.07.14.548955.
[PMID] 37503021.
2023
Production and characterization of an AAV1-VP3-only capsid: An analytical benchmark standard.
Molecular therapy. Methods & clinical development.
29:460-472
[DOI] 10.1016/j.omtm.2023.05.002.
[PMID] 37273903.
2023
Structural and antigenic characterization of the avian adeno-associated virus capsid
Journal of Virology.
97(10)
[DOI] 10.1128/jvi.00780-23.
[PMID] 37702486.
2023
Structural and Biophysical Analysis of Adeno-Associated Virus Serotype 2 Capsid Assembly Variants.
Journal of virology.
97(7)
[DOI] 10.1128/jvi.01772-22.
[PMID] 37310260.
2023
Structural and functional characterization of capsid binding by anti-AAV9 monoclonal antibodies from infants after SMA gene therapy.
Molecular therapy : the journal of the American Society of Gene Therapy.
31(7):1979-1993
[DOI] 10.1016/j.ymthe.2023.03.032.
[PMID] 37012705.
2023
Structural Characterization of Canine Minute Virus, Rat and Porcine Bocavirus
Viruses.
15(9)
[DOI] 10.3390/v15091799.
[PMID] 37766206.
2022
Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait.
Viruses.
14(10)
[DOI] 10.3390/v14102219.
[PMID] 36298773.
2022
Characterization of the Serpentine Adeno-Associated Virus (SAAV) Capsid Structure: Receptor Interactions and Antigenicity.
Journal of virology.
96(11)
[DOI] 10.1128/jvi.00335-22.
[PMID] 35532224.
2022
Structural characterization of an envelope-associated adeno-associated virus type 2 capsid.
Virology.
565:22-28
[DOI] 10.1016/j.virol.2021.09.010.
[PMID] 34638006.
2022
Structurally Mapping Antigenic Epitopes of Adeno-associated Virus 9: Development of Antibody Escape Variants.
Journal of virology.
96(3)
[DOI] 10.1128/JVI.01251-21.
[PMID] 34757842.
2021
Adeno-associated Virus (AAV) Capsid Chimeras with Enhanced Infectivity Reveal a Core Element in the AAV Genome Critical for both Cell Transduction and Capsid Assembly.
Journal of virology.
95(7)
[DOI] 10.1128/JVI.02023-20.
[PMID] 33441336.
2021
Characterization of the GBoV1 Capsid and Its Antibody Interactions.
Viruses.
13(2)
[DOI] 10.3390/v13020330.
[PMID] 33672786.
2021
Comparative structural, biophysical, and receptor binding study of true type and wild type AAV2.
Journal of structural biology.
213(4)
[DOI] 10.1016/j.jsb.2021.107795.
[PMID] 34509611.
2021
Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features.
Viruses.
13(1)
[DOI] 10.3390/v13010101.
[PMID] 33450892.
2021
Improved Genome Packaging Efficiency of Adeno-associated Virus Vectors Using Rep Hybrids.
Journal of virology.
95(19)
[DOI] 10.1128/JVI.00773-21.
[PMID] 34287038.
2021
Parvovirus Capsid-Antibody Complex Structures Reveal Conservation of Antigenic Epitopes Across the Family.
Viral immunology.
34(1):3-17
[DOI] 10.1089/vim.2020.0022.
[PMID] 32315582.
2021
pH-Induced Conformational Changes of Human Bocavirus Capsids.
Journal of virology.
95(8)
[DOI] 10.1128/JVI.02329-20.
[PMID] 33472934.
2021
Receptor Switching in Newly Evolved Adeno-associated Viruses.
Journal of virology.
95(19)
[DOI] 10.1128/JVI.00587-21.
[PMID] 34232726.
2021
Structural Study of Aavrh.10 Receptor and Antibody Interactions.
Journal of virology.
95(23)
[DOI] 10.1128/JVI.01249-21.
[PMID] 34549984.
2020
Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking.
Viruses.
12(6)
[DOI] 10.3390/v12060668.
[PMID] 32575696.
2020
Characterization of AAV-Specific Affinity Ligands: Consequences for Vector Purification and Development Strategies.
Molecular therapy. Methods & clinical development.
19:362-373
[DOI] 10.1016/j.omtm.2020.10.001.
[PMID] 33145372.
2020
Coevolution of Adeno-associated Virus Capsid Antigenicity and Tropism through a Structure-Guided Approach.
Journal of virology.
94(19)
[DOI] 10.1128/JVI.00976-20.
[PMID] 32669336.
2020
Comparative Analysis of the Capsid Structures of AAVrh.10, AAVrh.39, and AAV8.
Journal of virology.
94(6)
[DOI] 10.1128/JVI.01769-19.
[PMID] 31826994.
2020
Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome Editing.
Molecular therapy. Nucleic acids.
20:451-458
[DOI] 10.1016/j.omtn.2020.03.009.
[PMID] 32276210.
2020
Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity.
Journal of virology.
94(11)
[DOI] 10.1128/JVI.00170-20.
[PMID] 32213611.
2020
Structural characterization of a bat Adeno-associated virus capsid.
Journal of structural biology.
211(2)
[DOI] 10.1016/j.jsb.2020.107547.
[PMID] 32522552.
2020
Structural Characterization of Cuta- and Tusavirus: Insight into Protoparvoviruses Capsid Morphology.
Viruses.
12(6)
[DOI] 10.3390/v12060653.
[PMID] 32560452.
2019
Dynorphin-based “release on demand” gene therapy for drug-resistant temporal lobe epilepsy.
EMBO molecular medicine.
11(10)
[DOI] 10.15252/emmm.201809963.
[PMID] 31486590.
2019
High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors.
Journal of virology.
93(1)
[DOI] 10.1128/JVI.01394-18.
[PMID] 30333169.
2019
ICTV Virus Taxonomy Profile: Parvoviridae.
The Journal of general virology.
100(3):367-368
[DOI] 10.1099/jgv.0.001212.
[PMID] 30672729.
2019
Twenty-Five Years of Structural Parvovirology.
Viruses.
11(4)
[DOI] 10.3390/v11040362.
[PMID] 31010002.
2018
Atomic Resolution Structures of Human Bufaviruses Determined by Cryo-Electron Microscopy.
Viruses.
10(1)
[DOI] 10.3390/v10010022.
[PMID] 29300333.
2018
Atomic structure of a rationally engineered gene delivery vector, AAV2.5.
Journal of structural biology.
203(3):236-241
[DOI] 10.1016/j.jsb.2018.05.004.
[PMID] 29775653.
2018
CART neurons in the arcuate nucleus and lateral hypothalamic area exert differential controls on energy homeostasis.
Molecular metabolism.
7:102-118
[DOI] 10.1016/j.molmet.2017.10.015.
[PMID] 29146410.
2018
Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.
Nature communications.
9(1)
[DOI] 10.1038/s41467-018-06076-6.
[PMID] 30194371.
2017
Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells.
Molecular therapy : the journal of the American Society of Gene Therapy.
25(12):2661-2675
[DOI] 10.1016/j.ymthe.2017.08.003.
[PMID] 28890324.
2017
OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA.
Human gene therapy methods.
28(1):15-22
[DOI] 10.1089/hgtb.2016.164.
[PMID] 28125901.
2017
Structural Insights into Human Bocaparvoviruses.
Journal of virology.
91(11)
[DOI] 10.1128/JVI.00261-17.
[PMID] 28331084.
2017
The Good That Viruses Do.
Annual review of virology.
4(1):iii-v
[DOI] 10.1146/annurev-vi-04-071217-100011.
[PMID] 28961414.
2017
Thermal Stability as a Determinant of AAV Serotype Identity.
Molecular therapy. Methods & clinical development.
6:171-182
[DOI] 10.1016/j.omtm.2017.07.003.
[PMID] 28828392.
2017
Understanding capsid assembly and genome packaging for adeno-associated viruses.
Future virology.
12(6):283-297
[DOI] 10.2217/fvl-2017-0011.
[PMID] 36776482.
2016
A Comprehensive RNA Sequencing Analysis of the Adeno-Associated Virus (AAV) Type 2 Transcriptome Reveals Novel AAV Transcripts, Splice Variants, and Derived Proteins.
Journal of virology.
90(3):1278-89
[DOI] 10.1128/JVI.02750-15.
[PMID] 26559843.
2016
Comprehensive Small RNA-Seq of Adeno-Associated Virus (AAV)-Infected Human Cells Detects Patterns of Novel, Non-Coding AAV RNAs in the Absence of Cellular miRNA Regulation.
PloS one.
11(9)
[DOI] 10.1371/journal.pone.0161454.
[PMID] 27611072.
2015
NPY Y2 receptors in the central amygdala reduce cued but not contextual fear
Neuropharmacology.
99:665-674
[DOI] 10.1016/j.neuropharm.2015.08.038.
2015
OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA.
Human gene therapy.
26(10):688-97
[DOI] 10.1089/hum.2015.050.
[PMID] 26134901.
2014
A simplified purification protocol for recombinant adeno-associated virus vectors.
Molecular therapy. Methods & clinical development.
1
[DOI] 10.1038/mtm.2014.34.
[PMID] 26015974.
2014
Differential adeno-associated virus serotype-specific interaction patterns with synthetic heparins and other glycans.
Journal of virology.
88(5):2991-3003
[DOI] 10.1128/JVI.03371-13.
[PMID] 24371066.
2014
OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.
Human gene therapy.
25(3):212-22
[DOI] 10.1089/hum.2013.184.
[PMID] 24299301.
2013
Arcuate NPY controls sympathetic output and BAT function via a relay of tyrosine hydroxylase neurons in the PVN.
Cell metabolism.
17(2):236-48
[DOI] 10.1016/j.cmet.2013.01.006.
[PMID] 23395170.
2012
DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8.
Journal of virology.
86(5):2859-63
[DOI] 10.1128/JVI.06364-11.
[PMID] 22205745.
Grants
Apr 2024
– Apr 2024
Structural characterization of AAV capsid ligand complexes
Role: Co-Project Director/Principal Investigator
Funding: AVITIDE
Jan 2024
– Jan 2024
Structural characterization of AAV capsid ligand complexes
Role: Co-Project Director/Principal Investigator
Funding: AVITIDE
Jan 2024
ACTIVE
A modified arrestin1 to enhance glycolysis in photoreceptors as a therapeutic approach to slowing retinal degeneration
Role: Co-Investigator
Funding: NATL INST OF HLTH NEI
Nov 2022
– Oct 2023
A study into the expression of TTV and its possible development as a gene therapy vector
Role: Co-Investigator
Funding: INTIMA BIOSCIENCE
Feb 2022
– Jul 2022
Cryo-EM analysis of AAV capsid full/empty ratio determinations
Role: Co-Investigator
Funding: STRIDEBIO
Dec 2021
– Jan 2022
Generation of AAV1 VP3-only DNA empty capsids
Role: Co-Investigator
Funding: THERMO FINNIGAN
Aug 2021
– Oct 2021
Generation of AAV1 VP3 only DNA empty capsids
Role: Co-Investigator
Funding: THERMO FINNIGAN
Jun 2018
– Apr 2022
A role for AAV Rep in genome packaging efficiency
Role: Co-Investigator
Funding: INTIMA BIOSCIENCE
Jan 2017
– May 2022
AAV1 Variants for CNS Targeted Therapies
Role: Co-Investigator
Funding: VOYAGER THERAPEUTICS INC
Contact Details
Phones:
- Business:
- (352) 846-1836
Emails:
- Business:
- mario.mietzsch@ufl.edu
Addresses:
- Business Mailing:
-
PO BOX 100245
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIO
GAINESVILLE FL 326100245