Mario Mietzsch

Mario Mietzsch,

AST SCTST

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 846-1836
Business Email: mario.mietzsch@ufl.edu

Teaching Profile

Courses Taught
2020-2022
GMS6251 Molecular Therapy I ? Vectors and Molecular Mechanisms
2021
BCH6747 Structural Biology/Advanced Physical Biochemistry: Spectroscopy and Hydrodynamics
2021-2022
BCH6740 Physical Biochemistry/Structural Biology

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0001-8957-3363

Publications

2022
Characterization of the Serpentine Adeno-Associated Virus (SAAV) Capsid Structure: Receptor Interactions and Antigenicity.
Journal of virology. 96(11) [DOI] 10.1128/jvi.00335-22. [PMID] 35532224.
2022
Structural characterization of an envelope-associated adeno-associated virus type 2 capsid.
Virology. 565:22-28 [DOI] 10.1016/j.virol.2021.09.010. [PMID] 34638006.
2022
Structurally Mapping Antigenic Epitopes of Adeno-associated Virus 9: Development of Antibody Escape Variants.
Journal of virology. 96(3) [DOI] 10.1128/JVI.01251-21. [PMID] 34757842.
2021
Adeno-associated Virus (AAV) Capsid Chimeras with Enhanced Infectivity Reveal a Core Element in the AAV Genome Critical for both Cell Transduction and Capsid Assembly.
Journal of virology. 95(7) [DOI] 10.1128/JVI.02023-20. [PMID] 33441336.
2021
Characterization of the GBoV1 Capsid and Its Antibody Interactions.
Viruses. 13(2) [DOI] 10.3390/v13020330. [PMID] 33672786.
2021
Comparative structural, biophysical, and receptor binding study of true type and wild type AAV2.
Journal of structural biology. 213(4) [DOI] 10.1016/j.jsb.2021.107795. [PMID] 34509611.
2021
Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features.
Viruses. 13(1) [DOI] 10.3390/v13010101. [PMID] 33450892.
2021
Improved Genome Packaging Efficiency of Adeno-associated Virus Vectors Using Rep Hybrids.
Journal of virology. 95(19) [DOI] 10.1128/JVI.00773-21. [PMID] 34287038.
2021
Parvovirus Capsid-Antibody Complex Structures Reveal Conservation of Antigenic Epitopes Across the Family.
Viral immunology. 34(1):3-17 [DOI] 10.1089/vim.2020.0022. [PMID] 32315582.
2021
pH-Induced Conformational Changes of Human Bocavirus Capsids.
Journal of virology. 95(8) [DOI] 10.1128/JVI.02329-20. [PMID] 33472934.
2021
Receptor Switching in Newly Evolved Adeno-associated Viruses.
Journal of virology. 95(19) [DOI] 10.1128/JVI.00587-21. [PMID] 34232726.
2021
Structural Study of Aavrh.10 Receptor and Antibody Interactions.
Journal of virology. 95(23) [DOI] 10.1128/JVI.01249-21. [PMID] 34549984.
2020
Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking.
Viruses. 12(6) [DOI] 10.3390/v12060668. [PMID] 32575696.
2020
Characterization of AAV-Specific Affinity Ligands: Consequences for Vector Purification and Development Strategies.
Molecular therapy. Methods & clinical development. 19:362-373 [DOI] 10.1016/j.omtm.2020.10.001. [PMID] 33145372.
2020
Coevolution of Adeno-associated Virus Capsid Antigenicity and Tropism through a Structure-Guided Approach.
Journal of virology. 94(19) [DOI] 10.1128/JVI.00976-20. [PMID] 32669336.
2020
Comparative Analysis of the Capsid Structures of AAVrh.10, AAVrh.39, and AAV8.
Journal of virology. 94(6) [DOI] 10.1128/JVI.01769-19. [PMID] 31826994.
2020
Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome Editing.
Molecular therapy. Nucleic acids. 20:451-458 [DOI] 10.1016/j.omtn.2020.03.009. [PMID] 32276210.
2020
Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity.
Journal of virology. 94(11) [DOI] 10.1128/JVI.00170-20. [PMID] 32213611.
2020
Structural characterization of a bat Adeno-associated virus capsid.
Journal of structural biology. 211(2) [DOI] 10.1016/j.jsb.2020.107547. [PMID] 32522552.
2020
Structural Characterization of Cuta- and Tusavirus: Insight into Protoparvoviruses Capsid Morphology.
Viruses. 12(6) [DOI] 10.3390/v12060653. [PMID] 32560452.
2019
Dynorphin-based “release on demand” gene therapy for drug-resistant temporal lobe epilepsy.
EMBO molecular medicine. 11(10) [DOI] 10.15252/emmm.201809963. [PMID] 31486590.
2019
High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors.
Journal of virology. 93(1) [DOI] 10.1128/JVI.01394-18. [PMID] 30333169.
2019
ICTV Virus Taxonomy Profile: Parvoviridae.
The Journal of general virology. 100(3):367-368 [DOI] 10.1099/jgv.0.001212. [PMID] 30672729.
2019
Twenty-Five Years of Structural Parvovirology.
Viruses. 11(4) [DOI] 10.3390/v11040362. [PMID] 31010002.
2018
Atomic Resolution Structures of Human Bufaviruses Determined by Cryo-Electron Microscopy.
Viruses. 10(1) [DOI] 10.3390/v10010022. [PMID] 29300333.
2018
Atomic structure of a rationally engineered gene delivery vector, AAV2.5.
Journal of structural biology. 203(3):236-241 [DOI] 10.1016/j.jsb.2018.05.004. [PMID] 29775653.
2018
CART neurons in the arcuate nucleus and lateral hypothalamic area exert differential controls on energy homeostasis.
Molecular metabolism. 7:102-118 [DOI] 10.1016/j.molmet.2017.10.015. [PMID] 29146410.
2018
Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.
Nature communications. 9(1) [DOI] 10.1038/s41467-018-06076-6. [PMID] 30194371.
2017
Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells.
Molecular therapy : the journal of the American Society of Gene Therapy. 25(12):2661-2675 [DOI] 10.1016/j.ymthe.2017.08.003. [PMID] 28890324.
2017
OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA.
Human gene therapy methods. 28(1):15-22 [DOI] 10.1089/hgtb.2016.164. [PMID] 28125901.
2017
Structural Insights into Human Bocaparvoviruses.
Journal of virology. 91(11) [DOI] 10.1128/JVI.00261-17. [PMID] 28331084.
2017
The Good That Viruses Do.
Annual review of virology. 4(1):iii-v [DOI] 10.1146/annurev-vi-04-071217-100011. [PMID] 28961414.
2017
Thermal Stability as a Determinant of AAV Serotype Identity.
Molecular therapy. Methods & clinical development. 6:171-182 [DOI] 10.1016/j.omtm.2017.07.003. [PMID] 28828392.
2016
A Comprehensive RNA Sequencing Analysis of the Adeno-Associated Virus (AAV) Type 2 Transcriptome Reveals Novel AAV Transcripts, Splice Variants, and Derived Proteins.
Journal of virology. 90(3):1278-89 [DOI] 10.1128/JVI.02750-15. [PMID] 26559843.
2016
Comprehensive Small RNA-Seq of Adeno-Associated Virus (AAV)-Infected Human Cells Detects Patterns of Novel, Non-Coding AAV RNAs in the Absence of Cellular miRNA Regulation.
PloS one. 11(9) [DOI] 10.1371/journal.pone.0161454. [PMID] 27611072.
2015
NPY Y2 receptors in the central amygdala reduce cued but not contextual fear
Neuropharmacology. 99:665-674 [DOI] 10.1016/j.neuropharm.2015.08.038.
2015
OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA.
Human gene therapy. 26(10):688-97 [DOI] 10.1089/hum.2015.050. [PMID] 26134901.
2014
A simplified purification protocol for recombinant adeno-associated virus vectors.
Molecular therapy. Methods & clinical development. 1 [DOI] 10.1038/mtm.2014.34. [PMID] 26015974.
2014
Differential adeno-associated virus serotype-specific interaction patterns with synthetic heparins and other glycans.
Journal of virology. 88(5):2991-3003 [DOI] 10.1128/JVI.03371-13. [PMID] 24371066.
2014
OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.
Human gene therapy. 25(3):212-22 [DOI] 10.1089/hum.2013.184. [PMID] 24299301.
2013
Arcuate NPY controls sympathetic output and BAT function via a relay of tyrosine hydroxylase neurons in the PVN.
Cell metabolism. 17(2):236-48 [DOI] 10.1016/j.cmet.2013.01.006. [PMID] 23395170.
2012
DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8.
Journal of virology. 86(5):2859-63 [DOI] 10.1128/JVI.06364-11. [PMID] 22205745.
Sub-2 Å Ewald Curvature Corrected Single-Particle Cryo-EM
. [DOI] 10.1101/305599.

Grants

Nov 2022 ACTIVE
A study into the expression of TTV and its possible development as a gene therapy vector
Role: Co-Investigator
Funding: INTIMA BIOSCIENCE
Feb 2022 – Jul 2022
Cryo-EM analysis of AAV capsid full/empty ratio determinations
Role: Co-Investigator
Funding: STRIDEBIO
Dec 2021 – Jan 2022
Generation of AAV1 VP3-only DNA empty capsids
Role: Co-Investigator
Funding: THERMO FINNIGAN
Aug 2021 – Oct 2021
Generation of AAV1 VP3 only DNA empty capsids
Role: Co-Investigator
Funding: THERMO FINNIGAN
Jun 2018 – Apr 2022
A role for AAV Rep in genome packaging efficiency
Role: Co-Investigator
Funding: INTIMA BIOSCIENCE
Jan 2017 – May 2022
AAV1 Variants for CNS Targeted Therapies
Role: Co-Investigator
Funding: VOYAGER THERAPEUTICS INC

Contact Details

Phones:
Business:
(352) 846-1836
Emails:
Addresses:
Business Mailing:
PO BOX 100245
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIO
GAINESVILLE FL 326100245