Daniel L Purich

Daniel L Purich,

PROFESSOR

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 294-8400
Business Email: dlpurich@ufl.edu

Teaching Profile

Courses Taught
2013,2020
BCH6208 Advanced Metabolism: Regulation of Key Reactions in Carbohydrate and Lipid Metabolism
2016-2023
GMS5905 Special Topics in Biomedical Sciences
2013-2014,2019-2023
BMS6031 Foundations of Med
2013-2023
BCH4024 Introduction to Biochemistry and Molecular Biology
2013,2015-2020,2020-2023,2022-2023
BCH6207 Advanced Metabolism: Role of Membranes in Signal Transduction and Metabolic Control
2015-2023
MDU4010 Cell Biology Seminar
2013-2018,2020-2023
BCH6206 Advanced Metabolism
2011-2012,2014-2021,2020-2022
DEN5121 Biochm Molec Cell Bio
2007-2012,2015-2022
BCH7515 Structural Biology/Advanced Physical Biochemistry: Kinetics and Thermodynamics
2007-2019,2021-2022
BCH6740 Physical Biochemistry/Structural Biology
2018
GMS7979 Advanced Research
2018
GMS7980 Research for Doctoral Dissertation
2013-2015,2017-2023,2021-2023,2023
BCH4905 Biochemistry Senior Research
2014-2016
BCH6746 Structural Biology: Macromolecular Structure Determination
2007-2016
BCH6878 Recent Advances in Cytoskeletal Processes
2014-2015,2022-2023
BCH6209 Advanced Metabolism: Regulation of Key Reactions in Amino Acid and Nucleotide Metabolism
2013-2014
GMS6001 Fundamentals of Biomedical Sciences I

Publications

2016
‘Rod and ring’ formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.
Journal of cell science. 129(15):3042-52 [DOI] 10.1242/jcs.183400. [PMID] 27343244.
2016
Actoclampin (+)-end-tracking motors: How the pursuit of profilin’s role(s) in actin-based motility twice led to the discovery of how cells crawl.
Biophysical chemistry. 209:41-55 [DOI] 10.1016/j.bpc.2015.10.008. [PMID] 26720287.
2016
Angiopoietin-like protein 8 (betatrophin) is a stress-response protein that down-regulates expression of adipocyte triglyceride lipase.
Biochimica et biophysica acta. 1861(2):130-137 [DOI] 10.1016/j.bbalip.2015.11.003. [PMID] 26569053.
2016
Chemosensitizing AML cells by targeting bone marrow endothelial cells.
Experimental hematology. 44(5):363-377.e5 [DOI] 10.1016/j.exphem.2016.02.003. [PMID] 26898708.
2015
Activation of the Neuroprotective Angiotensin-Converting Enzyme 2 in Rat Ischemic Stroke.
Hypertension (Dallas, Tex. : 1979). 66(1):141-8 [DOI] 10.1161/HYPERTENSIONAHA.115.05185. [PMID] 25941346.
2015
Ionic Functionalization of Hydrophobic Colloidal Nanoparticles To Form Ionic Nanoparticles with Enzymelike Properties.
Journal of the American Chemical Society. 137(47):14952-8 [DOI] 10.1021/jacs.5b08533. [PMID] 26562739.
2014
Glutamine deprivation initiates reversible assembly of mammalian rods and rings.
Cellular and molecular life sciences : CMLS. 71(15):2963-73 [DOI] 10.1007/s00018-014-1567-6. [PMID] 24477477.
2013
Genetically reprogrammed, liver-derived insulin-producing cells are glucose-responsive, but susceptible to autoimmune destruction in settings of murine model of type 1 diabetes.
American journal of translational research. 5(2):184-99 [PMID] 23573363.
2012
Regional citrate anticoagulation for slow continuous ultrafiltration: risk of severe metabolic alkalosis.
Clinical kidney journal. 5(3):212-6 [DOI] 10.1093/ckj/sfs045. [PMID] 26069766.
2008
Carbon monoxide and nitric oxide mediate cytoskeletal reorganization in microvascular cells via vasodilator-stimulated phosphoprotein phosphorylation: evidence for blunted responsiveness in diabetes.
Diabetes. 57(9):2488-94 [DOI] 10.2337/db08-0381. [PMID] 18559661.
2008
Reversal of streptozotocin-induced diabetes in mice by cellular transduction with recombinant pancreatic transcription factor pancreatic duodenal homeobox-1: a novel protein transduction domain-based therapy.
Diabetes. 57(3):757-69 [PMID] 18086901.
2007
Guidance of actin filament elongation on filament-binding tracks.
Langmuir : the ACS journal of surfaces and colloids. 23(23):11911-6 [PMID] 17929952.
2007
Nematode sperm motility: nonpolar filament polymerization mediated by end-tracking motors.
Biophysical journal. 92(2):622-31 [PMID] 17056726.
2006
Diffusion rate limitations in actin-based propulsion of hard and deformable particles.
Biophysical journal. 91(4):1548-63 [PMID] 16731556.
2005
Listeria’s right-handed helical rocket-tail trajectories: mechanistic implications for force generation in actin-based motility.
Cell motility and the cytoskeleton. 60(2):121-8 [PMID] 15627275.
2005
Structural insights into Alzheimer filament assembly pathways based on site-directed mutagenesis and S-glutathionylation of three-repeat neuronal Tau protein.
Microscopy research and technique. 67(3-4):156-63 [PMID] 16104002.
2004
Force generation by cytoskeletal filament end-tracking proteins.
Biophysical journal. 87(4):2838-54 [PMID] 15454475.
2003
Actin-based endosome and phagosome rocketing in macrophages: activation by the secretagogue antagonists lanthanum and zinc.
Cell motility and the cytoskeleton. 54(1):41-55 [PMID] 12451594.
2002
A direct-transfer polymerization model explains how the multiple profilin-binding sites in the actoclampin motor promote rapid actin-based motility.
Archives of biochemistry and biophysics. 406(2):296-301 [PMID] 12361718.
2002
A survey of covalent, ionic, and radical intermediates in enzyme-catalyzed reactions.
Methods in enzymology. 354:455-70 [PMID] 12418246.
2002
Actin-based phagosome motility.
Cell motility and the cytoskeleton. 53(2):81-8 [PMID] 12211106.
2002
Clamped-filament elongation model for actin-based motors.
Biophysical journal. 82(2):605-17 [PMID] 11806905.
2002
Covalent enzyme-substrate compounds: detection and catalytic competence.
Methods in enzymology. 354:1-27 [PMID] 12418214.
2002
In vitro assembly of Alzheimer-like filaments. How a small cluster of charged residues in Tau and MAP2 controls filament morphology.
The Journal of biological chemistry. 277(38):34755-9 [PMID] 12023276.
2002
Use of sodium borohydride to detect acyl-phosphate linkages in enzyme reactions.
Methods in enzymology. 354:168-77 [PMID] 12418224.
2001
Enzyme catalysis: a new definition accounting for noncovalent substrate- and product-like states.
Trends in biochemical sciences. 26(7):417-21 [PMID] 11440852.
2001
N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility.
Nature cell biology. 3(10):897-904 [PMID] 11584271.
2000
Shigella actin-based motility in the presence of truncated vinculin.
Cell motility and the cytoskeleton. 45(4):272-8 [PMID] 10744860.
1999
Actin-based motility of the intracellular pathogen Listeria monocytogenes: assessing the inhibitory specificity of ABM-1 peptide analogues.
Molecular cell biology research communications : MCBRC. 1(3):176-81 [PMID] 10425223.
1999
Disulfide-cross-linked tau and MAP2 homodimers readily promote microtubule assembly.
Molecular cell biology research communications : MCBRC. 2(1):71-6 [PMID] 10527895.
1999
Disulfide-linked MAP2-MAP2 Dimers Still Promote Tubulin Polymerization and Stabilize Microtubules
Molecular Cell Biology Research Communications. 2:71-76
1999
Energetics of nucleotide hydrolysis in polymer assembly/disassembly: the cases of actin and tubulin.
Methods in enzymology. 308:93-111 [PMID] 10507002.
1999
Profilin promotes barbed-end actin filament assembly without lowering the critical concentration.
The Journal of biological chemistry. 274(52):36963-72 [PMID] 10601251.
1998
Advances in the enzymology of glutamine synthesis.
Advances in enzymology and related areas of molecular biology. 72:9-42 [PMID] 9559050.
1998
Listeria and Shigella actin-based motility in host cells.
Transactions of the American Clinical and Climatological Association. 109:160-72; discussion 172 [PMID] 9601135.
1998
Vaccinia locomotion in host cells: evidence for the universal involvement of actin-based motility sequences ABM-1 and ABM-2.
Proceedings of the National Academy of Sciences of the United States of America. 95(23):13917-22 [PMID] 9811901.
1997
ABM-1 and ABM-2 homology sequences: consensus docking sites for actin-based motility defined by oligoproline regions in Listeria ActA surface protein and human VASP.
Biochemical and biophysical research communications. 231(3):686-91 [PMID] 9070872.
1997
Profilin interacts with the Gly-Pro-Pro-Pro-Pro-Pro sequences of vasodilator-stimulated phosphoprotein (VASP): implications for actin-based Listeria motility.
Biochemistry. 36(27):8384-92 [PMID] 9204886.
1997
Vinculin proteolysis unmasks an ActA homolog for actin-based Shigella motility.
The Journal of cell biology. 138(6):1255-64 [PMID] 9298981.
1996
Assembly of MAP-2c and MTBR Fragments into Polymers Resembling Paired Helical Filaments
Journal of Biological Chemistry. 271:32702-32706
1996
In vitro polymerization of embryonic MAP-2c and fragments of the MAP-2 microtubule binding region into structures resembling paired helical filaments.
The Journal of biological chemistry. 271(51):32702-6 [PMID] 8955102.
1996
Intracellular pathogenesis of listeriosis.
The New England journal of medicine. 334(12):770-6 [PMID] 8592552.
1996
Recognition of two classes of oligoproline sequences in profilin-mediated acceleration of actin-based Shigella motility.
The Journal of cell biology. 133(1):49-59 [PMID] 8601612.
1996
Self-assembly of the brain MAP-2 microtubule-binding region into polymeric structures resembling Alzheimer filaments.
Biochemical and biophysical research communications. 229(1):176-81 [PMID] 8954102.
1995
Inhibition of Listeria locomotion by mosquito oostatic factor, a natural oligoproline peptide uncoupler of profilin action.
Infection and immunity. 63(1):182-90 [PMID] 7806356.
1995
Non-cooperative binding of the MAP-2 microtubule-binding region to microtubules.
The Journal of biological chemistry. 270(3):1035-40 [PMID] 7836356.
1994
Arrest of Listeria movement in host cells by a bacterial ActA analogue: implications for actin-based motility.
Proceedings of the National Academy of Sciences of the United States of America. 91(11):5168-72 [PMID] 8197202.
1994
Calcium Oxalate Crystal Morphology: Influence of Phospholipid Micelles with Composition Based on Each Leaflet of the Erythrocyte Membrane
Journal of Crystal Growth. 135:523-532
1994
Dynamic remodeling of the actin cytoskeleton: lessons learned from Listeria locomotion.
BioEssays : news and reviews in molecular, cellular and developmental biology. 16(12):885-91 [PMID] 7840767.
1994
EQUIL93: a tool for experimental and clinical urolithiasis.
Urological research. 22(2):119-26 [PMID] 7974915.
1994
Exploring the microtubule-binding region of bovine microtubule-associated protein-2 (MAP-2): cDNA sequencing, bacterial expression, and site-directed mutagenesis.
Biochemistry. 33(45):13199-207 [PMID] 7947727.
1994
Microtubule Dynamics: Bioenergetics and Control
. 69:121-154
1994
Microtubule dynamics: bioenergetics and control.
Advances in enzymology and related areas of molecular biology. 69:121-54 [PMID] 7817867.
1994
Phosphorylation states of actin filament adenine nucleotides in detergent-extracted neuronal cytoskeletal fractions.
Biochemical and biophysical research communications. 201(3):1490-4 [PMID] 8024594.
1994
Stimulation of tubulin polymerization by MAP-2. Control by protein kinase C-mediated phosphorylation at specific sites in the microtubule-binding region.
The Journal of biological chemistry. 269(45):28465-71 [PMID] 7961787.
1993
Absence of 2′-deoxy-GTP in adult brain tubulin.
Biochemical and biophysical research communications. 192(1):268-72 [PMID] 8476429.
1993
Microtubule-associated protein interactions with actin filaments: evidence for differential behavior of neuronal MAP-2 and tau in the presence of phosphatidyl-inositol.
Biochemical and biophysical research communications. 190(3):710-5 [PMID] 8439322.
1993
Reduction in microtubule dynamics in vitro by brain microtubule-associated proteins and by a microtubule-associated protein-2 second repeated sequence analogue.
Journal of neurochemistry. 60(3):817-26 [PMID] 7679726.
1992
2′-Deoxy-GTP in the microtubule cytoskeleton of neuronal cells cultured with nerve growth factor.
The Journal of biological chemistry. 267(36):25685-9 [PMID] 1464585.
1992
Adenine and guanine nucleotide content of Triton-extracted cytoskeletal fractions of nonmuscle cells.
Analytical biochemistry. 204(1):47-52 [PMID] 1514695.
1992
ADPRibosylation of chicken red cell tubulin and inhibition of microtubule self-assembly in vitro by the NAD(+)-dependent avian ADPRibosyl transferase.
Biochemical and biophysical research communications. 184(1):414-8 [PMID] 1348935.
1992
Calcium Oxalate Monohydrate Crystallization: Citrate Inhibition of Nucleation and Growth Steps
Journal of Crystal Growth. 125:215-222
1992
Cleavage of bovine brain microtubule-associated protein-2 by human immunodeficiency virus proteinase.
Journal of neurochemistry. 59(3):874-80 [PMID] 1494913.
1992
Microtubule protein ADP-ribosylation in vitro leads to assembly inhibition and rapid depolymerization.
Biochemistry. 31(1):310-6 [PMID] 1731882.
1992
Microtubule-associated protein autophosphorylation alters in vitro microtubule dynamic instability.
FEBS letters. 296(1):21-4 [PMID] 1346116.
1992
Steady-state kinetic mechanism of bovine brain tubulin: tyrosine ligase.
The Biochemical journal. 286 ( Pt 1)(Pt 1):243-51 [PMID] 1520276.
1991
Evidence against impaired brain microtubule protein polymerization at high glucose concentrations or during diabetes mellitus.
Journal of neurochemistry. 56(6):2087-93 [PMID] 2027016.
1991
Strong inference in mechanistic urolithiasis: a tribute to Birdwell Finlayson’s biophysical contributions.
American journal of kidney diseases : the official journal of the National Kidney Foundation. 17(4):451-7 [PMID] 2008915.
1990
Apparently irreversible GTP hydrolysis attends tubulin self-assembly.
European journal of biochemistry. 191(2):507-11 [PMID] 2384097.
1990
Peptides corresponding to the second repeated sequence in MAP-2 inhibit binding of microtubule-associated proteins to microtubules.
Biochemistry. 29(38):8916-20 [PMID] 2271567.
1989
Measurement of urinary supersaturation, creation of standard supersaturations.
Urological research. 17(3):144-6 [PMID] 2749940.
1989
The microtubule-binding fragment of microtubule-associated protein-2: location of the protease-accessible site and identification of an assembly-promoting peptide.
The Journal of cell biology. 109(5):2289-94 [PMID] 2808529.
1989
Use of the computer program EQUIL to estimate pH in model solutions and human urine.
Urological research. 17(3):157-61 [PMID] 2749945.
1987
GTP regeneration influences interactions of microtubules, neurofilaments, and microtubule-associated proteins in vitro.
The Journal of biological chemistry. 262(32):15443-7 [PMID] 3680206.
1987
Modulation of microtubule assembly and stability by phosphatidylinositol action on microtubule-associated protein-2.
The Journal of biological chemistry. 262(7):3369-75 [PMID] 2880849.
1987
The 28,000 Mr microtubule-binding domain of microtubule-associated protein-2 also contains a neurofilament-binding site.
Biochemical and biophysical research communications. 148(3):1453-9 [PMID] 3120718.
1986
Microtubule-associated proteins. In vitro isolation versus in vivo function.
Annals of the New York Academy of Sciences. 466:410-2 [PMID] 3089110.
1986
Several metabolic factors governing the dynamics of microtubule assembly and disassembly.
Annals of the New York Academy of Sciences. 466:519-28 [PMID] 2942085.
1985
Ciliary dynein conformational changes as evidenced by the extrinsic fluorescent probe 8-anilino-1-naphthalenesulfonate.
Biochemistry. 24(26):7581-5 [PMID] 2936390.
1985
Microtubule cytoskeletal proteins as targets for covalent interconverting enzymes.
Current topics in cellular regulation. 27:107-16 [PMID] 4092489.
1985
Microtubule-associated proteins (MAPs): a monoclonal antibody to MAP 1 decorates microtubules in vitro but stains stress fibers and not microtubules in vivo.
Proceedings of the National Academy of Sciences of the United States of America. 82(5):1434-8 [PMID] 3883359.
1985
Taxol stabilization of microtubules in vitro: dynamics of tubulin addition and loss at opposite microtubule ends.
Biochemistry. 24(19):5254-62 [PMID] 2866793.
1984
Microtubule assembly: a review of progress, principles, and perspectives.
Advances in protein chemistry. 36:133-212 [PMID] 6382962.
1984
Osmotic Susceptibility and Diffusion Coefficient of Charged Bovine Serum Albumin
. 80:3469-3477
1983
Concerning the equilibrium exchange kinetics and mechanism of the sheep brain glutamine synthetase reaction.
Biochemical and biophysical research communications. 115(1):220-4 [PMID] 6137218.
1982
A brain microtubule protein preparation depleted of mitochondrial and synaptosomal components.
Methods in cell biology. 24:51-60 [PMID] 7098996.
1982
A reinvestigation of dynein ATPase kinetics and the inhibitory action of vanadate.
The Journal of biological chemistry. 257(12):6656-8 [PMID] 6211437.
1982
An automated method for defining microtubule length distributions.
Methods in cell biology. 24:133-44 [PMID] 7098985.
1982
Characterization of tubulin and microtubule-associated protein interactions with guanine nucleotides and their nonhydrolyzable analogs.
Methods in enzymology. 85 Pt B:416-33 [PMID] 6289045.
1982
Criteria for evaluating the catalytic competence of enzyme-substrate covalent compounds.
Methods in enzymology. 87:3-20 [PMID] 7176919.
1982
Microtubule disassembly: a quantitative kinetic approach for defining endwise linear depolymerization.
Methods in enzymology. 85 Pt B:439-50 [PMID] 7121281.
1982
Microtubule interactions with GDP provide evidence that assembly-disassembly properties depend on the method of brain microtubule protein isolation.
Biochemical and biophysical research communications. 105(4):1605-10 [PMID] 7103973.
1982
Nucleotide-dependent enzymes associated with microtubule systems.
Advances in enzymology and related areas of molecular biology. 53:113-61 [PMID] 6120628.
1981
A Quantitative Treatment of Reversible Monomer-Polymer Exchange Reactions with Microtubules and Other Biopolymers
Journal of Theoretical Biology. 92:85-96
1981
Oxygenation of hemoglobin. Correspondence of crystal and solution properties using diffusion coefficient measurements.
Journal of molecular biology. 147(4):583-95 [PMID] 7277502.
1981
Taxol induces microtubule assembly at low temperature.
Cell motility. 1(4):445-54 [PMID] 6129064.
1981
Time scale of microtubule length redistribution.
Archives of biochemistry and biophysics. 211(1):222-6 [PMID] 7305367.
1980
A rapid dilution cuvette for kinetic studies of microtubule disassembly.
Analytical biochemistry. 104(2):311-4 [PMID] 7004264.
1980
Adenosine triphosphatase activity of bovine brain microtubule protein.
The Journal of biological chemistry. 255(2):486-91 [PMID] 6444297.
1980
Assembly and disassembly properties of microtubules formed in the presence of GTP, 5′-guanylyl imidodiphosphate, and 5′-guanylyl methylenediphosphate.
The Journal of biological chemistry. 255(21):10532-6 [PMID] 6253478.
1980
Autophosphorylation of brain microtubule protein: evidence for endogenous protein kinase/phosphoprotein phosphatase cycling and multiple phosphorylation of a microtubule associated protein.
Biochemical and biophysical research communications. 92(1):89-94 [PMID] 6243939.
1980
Calcium ion induces endwise depolymerization of bovine brain microtubules.
The Journal of biological chemistry. 255(24):11853-6 [PMID] 7440572.
1980
Dynamics of linear protein polymer disassembly.
The Journal of biological chemistry. 255(18):8567-72 [PMID] 6893327.
1980
Isotope exchange methods for elucidating enzymic catalysis.
Methods in enzymology. 64:1-46 [PMID] 6990185.
1980
Mechanism of microtubule depolymerization. Correlation of rapid induced disassembly experiments with a kinetic model for endwise depolymerization.
The Journal of biological chemistry. 255(18):8560-6 [PMID] 7410377.
1980
Rings are not microtubule assembly intermediates: an analysis of the lag phase in GTP-dependent self-assembly of bovine brain tubulin.
Biochemical and biophysical research communications. 95(4):1885-9 [PMID] 7417347.
1979
A microtubule assembly/disassembly model based on drug effects and depolymerization kinetics after rapid dilution.
The Journal of biological chemistry. 254(21):10885-8 [PMID] 500613.
1979
Characterization of brain microtubule proteins prepared by selective removal of mitochondrial and synaptosomal components.
The Journal of biological chemistry. 254(13):6107-11 [PMID] 447696.
1979
Nucleotide release from tubulin and nucleoside-5′-diphosphate kinase action in microtubule assembly.
The Journal of biological chemistry. 254(19):9469-76 [PMID] 226518.
1979
Participation of guanine nucleotides in nucleation and elongation steps of microtubule assembly.
Proceedings of the National Academy of Sciences of the United States of America. 76(11):5475-9 [PMID] 293656.
1979
Practical considerations in the design of initial velocity enzyme rate assays.
Methods in enzymology. 63:3-22 [PMID] 41157.
1978
Chromium (III)-nucleotide complexes as probes of the guanosine 5′-triphosphate-induced microtubule assembly.
Archives of biochemistry and biophysics. 191(1):233-43 [PMID] 736563.
1978
Examination of tubulin-nucleotide interactions by protein fluorescence quenching measurements.
Biochemical and biophysical research communications. 84(4):957-61 [PMID] 728162.
1978
Properties of tubulin treated with alkaline phosphatase to remove guanine nucleotides from the exchangeable binding site.
FEBS letters. 96(1):83-6 [PMID] 729795.
1978
Stoichiometry and role of GTP hydrolysis in bovine neurotubule assembly.
The Journal of biological chemistry. 253(13):4683-7 [PMID] 659441.
1977
Autophosphorylation of cardiac 3′,5′-cyclic AMP-stimulated protein kinase. Kinetic evidence for the regulatory subunit directly acting at the active site in the R2C2 complex.
Biochimica et biophysica acta. 485(1):87-94 [PMID] 911867.
1977
Neurotubule assembly at substoichiometric nucleotide levels using a GTP regenerating system.
Biochemical and biophysical research communications. 74(2):440-7 [PMID] 836300.
1977
On the rapid equilibrium assumption and the problem of distinguishing certain ordered and random enzyme kinetic mechanisms.
Biochemical and biophysical research communications. 77(2):753-9 [PMID] 901501.
1977
On the role of the tubulin nonexchangeable GTP site in bovine neurotubule assembly.
The Journal of biological chemistry. 252(13):4440-2 [PMID] 194881.
1977
Steady state and equilibrium exchange kinetic studies of the sheep brain glutamine synthetase reaction.
The Journal of biological chemistry. 252(17):6046-51 [PMID] 19464.
1976
A kinetically important phosphoryl-enzyme intermediary in the intrinsic purine nucleoside-5′-diphosphokinase activity of Escherichia coli acetate kinase.
Archives of biochemistry and biophysics. 174(1):120-8 [PMID] 180890.
1976
Studies on the kinetic mechanism and the phosphoryl-enzyme compound of the Escherichia coli acetate kinase reaction.
Archives of biochemistry and biophysics. 173(1):282-92 [PMID] 176952.
1975
Cascade control of Escherichia coli glutamine synthetase. Properties of the PII regulatory protein and the uridylyltransferase-uridylyl-removing enzyme.
The Journal of biological chemistry. 250(16):6264-72 [PMID] 239942.
1975
Omega-aminoalkyl agaroses in the resolution of enzymes involved in regulation of glutamine metabolism.
Proceedings of the National Academy of Sciences of the United States of America. 72(9):3397-401 [PMID] 242003.
1975
Regulation of Escherichia coli glutamine synthetase. Evidence for the action of some feedback modifiers at the active site of the unadenylylated enzyme.
Biochemistry. 14(9):1980-9 [PMID] 235974.
1975
Use of the sodium borohydride reduction technique to identify a gamma-glutamyl phosphate intermediary in the Escherichia coli glutamine synthetase reaction.
The Journal of biological chemistry. 250(9):3505-9 [PMID] 235549.
1974
5′-Adenylylation and 5′-uridylation of N-acetyl tyrosine ethyl ester: a general synthesis of 5′-nucleotidylated tyrosyl residues.
Biochemical and biophysical research communications. 59(1):82-5 [PMID] 4366954.
1974
Evidence for the formation of a gamma-phosphorylated glutamyl residue in the Escherichia coli acetate kinase reaction.
Biochemical and biophysical research communications. 60(1):273-80 [PMID] 4370922.
1973
Additional factors influencing enzyme responses to the adenylate energy charge.
The Journal of biological chemistry. 248(2):461-6 [PMID] 4265285.
1973
The hexokinases: kinetic, physical, and regulatory properties.
Advances in enzymology and related areas of molecular biology. 39:249-326 [PMID] 4583639.
1972
Activation of brain hexokinase by magnesium ions and by magnesium ion–adenosine triphosphate complex.
The Biochemical journal. 130(1):63-9 [PMID] 4655453.
1972
Application of equilibrium exchange kinetics to studies of kinetic properties at high enzyme concentrations: experiments with yeast hexokinase P-II.
Biochemical and biophysical research communications. 47(4):916-22 [PMID] 5026301.
1972
Evaluation of the phosphoryl-enzyme intermediate concept in the acetate kinase and hexokinase reactions from kinetic studies.
Archives of biochemistry and biophysics. 149(1):307-15 [PMID] 4552801.
1972
Inhibition of rabbit skeletal muscle adenylate kinase by the transition state analogue, P 1 ,P 4 -di(adenosine-5′)tetraphosphate.
Biochimica et biophysica acta. 276(2):563-7 [PMID] 5068828.
1972
Limitations in the use of Dixon plots to evaluate enzyme inhibition.
Biochimica et biophysica acta. 268(1):1-3 [PMID] 4622931.
1972
Studies on factors influencing enzyme responses to adenylate energy charge.
The Journal of biological chemistry. 247(1):249-55 [PMID] 5017764.
1971
The kinetics and regulation of rat brain hexokinase.
The Journal of biological chemistry. 246(11):3456-63 [PMID] 5578903.
1969
Studies on the kinetic mechanism and allosteric nature of bovine brain hexokinase.
The Journal of biological chemistry. 244(14):3840-6 [PMID] 4308737.
1968
Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. I. Partial purification, properties, and kinetic studies of the enzyme.
The Journal of biological chemistry. 243(21):5539-45 [PMID] 4301680.

Contact Details

Phones:
Business:
(352) 294-8400
Emails:
Business:
dlpurich@ufl.edu
Addresses:
Business Mailing:
PO Box 100245
GAINESVILLE FL 32610
Business Street:
R3126 ARB
GAINESVILLE FL 32611