Joanna R Long

Joanna R Long,

Professor

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 294-8399
Business Email: jrlong@ufl.edu

Teaching Profile

Courses Taught
2013,2015-2017,2017-2021,2019-2023,2021-2023,2023
BCH6745L Molecular Structure and Dynamics by NMR Spectroscopy Laboratory
2013,2015-2017,2017-2021,2019-2023,2021-2023,2023
BCH6745 Molecular Structure and Dynamics of NMR Spectroscopy
2014-2019,2021-2022
BCH6740 Physical Biochemistry/Structural Biology
2014-2019,2021-2022
BCH6746 Structural Biology: Macromolecular Structure Determination
2018
GMS7980 Research for Doctoral Dissertation
2018
CHM7980 Research for Doctoral Dissertation
2018
CHM7979 Advanced Research
2015,2021
BCH4905 Biochemistry Senior Research
2010
IDH4917 Undergrad Research

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0002-5572-6859

Publications

2022
Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase α-aminoacrylate intermediate.
Proceedings of the National Academy of Sciences of the United States of America. 119(2) [DOI] 10.1073/pnas.2109235119. [PMID] 34996869.
2021
Organic Matter Chemistry Drives Carbon Dioxide Production of Peatlands
Geophysical Research Letters. 48(18) [DOI] 10.1029/2021gl093392.
2020
Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking.
Viruses. 12(6) [DOI] 10.3390/v12060668. [PMID] 32575696.
2020
Characterization of an intermolecular quaternary interaction between discrete segments of theStreptococcus mutansadhesin P1 by NMR spectroscopy
The FEBS Journal. 287(12):2597-2611 [DOI] 10.1111/febs.15158. [PMID] 31782893.
2020
Comparison of Multivendor Single-Voxel MR Spectroscopy Data Acquired in Healthy Brain at 26 Sites.
Radiology. 295(1):171-180 [DOI] 10.1148/radiol.2020191037. [PMID] 32043950.
2020
Dynamic Nuclear Polarization of Biomembrane Assemblies.
Biomolecules. 10(9) [DOI] 10.3390/biom10091246. [PMID] 32867275.
2020
Enhanced purification coupled with biophysical analyses shows cross-β structure as a core building block for Streptococcus mutans functional amyloids.
Scientific reports. 10(1) [DOI] 10.1038/s41598-020-62115-7. [PMID] 32198417.
2019
Characterization of Brain Metabolism by Nuclear Magnetic Resonance.
Chemphyschem : a European journal of chemical physics and physical chemistry. 20(2):216-230 [DOI] 10.1002/cphc.201800917. [PMID] 30536696.
2019
Direct dynamic nuclear polarization of 15N and 13C spins at 14.1 T using a trityl radical and magic angle spinning
Solid State Nuclear Magnetic Resonance. 100:85-91 [DOI] 10.1016/j.ssnmr.2019.03.009. [PMID] 31026722.
2017
Chemical Composition of Soil Organic Matter in a Subarctic Peatland: Influence of Shifting Vegetation Communities
Soil Science Society of America Journal. 81(1):41-49 [DOI] 10.2136/sssaj2016.05.0148.
2017
Entropic Anomaly Observed in Lipid Polymorphisms Induced by Surfactant Peptide SP-B(1–25)
The Journal of Physical Chemistry B. 121(39):9102-9112 [DOI] 10.1021/acs.jpcb.7b06538.
2017
Functional amyloids in Streptococcus mutans, their use as targets of biofilm inhibition and initial characterization of SMU_63c.
Microbiology (Reading, England). 163(4):488-501 [DOI] 10.1099/mic.0.000443. [PMID] 28141493.
2016
Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy.
Analytical chemistry. 88(1):122-32 [DOI] 10.1021/acs.analchem.5b04376. [PMID] 26594903.
2016
Expeditious dissolution dynamic nuclear polarization without glassing agents.
NMR in biomedicine. 29(3):226-31 [DOI] 10.1002/nbm.3473. [PMID] 26915792.
2016
Molecular Rationale for Improved Dynamic Nuclear Polarization of Biomembranes.
The journal of physical chemistry. B. 120(32):7880-8 [DOI] 10.1021/acs.jpcb.6b02885. [PMID] 27434371.
2016
Peptide-based synthetic pulmonary surfactant for the treatment of respiratory distress disorders.
Current opinion in chemical biology. 32:22-8 [DOI] 10.1016/j.cbpa.2016.02.012. [PMID] 26970670.
2016
Specific binding of a naturally occurring amyloidogenic fragment of Streptococcus mutans adhesin P1 to intact P1 on the cell surface characterized by solid state NMR spectroscopy.
Journal of biomolecular NMR. 64(2):153-64 [DOI] 10.1007/s10858-016-0017-1. [PMID] 26837620.
2015
A method for dynamic nuclear polarization enhancement of membrane proteins.
Angewandte Chemie (International ed. in English). 54(5):1542-6 [DOI] 10.1002/anie.201410249. [PMID] 25504310.
2015
Delineation of the dynamic properties of individual lipid species in native and synthetic pulmonary surfactants
Biochimica et Biophysica Acta (BBA) – Biomembranes. 1848(1):203-210 [DOI] 10.1016/j.bbamem.2014.05.012.
2014
An intramolecular lock facilitates folding and stabilizes the tertiary structure of Streptococcus mutans adhesin P1.
Proceedings of the National Academy of Sciences of the United States of America. 111(44):15746-51 [DOI] 10.1073/pnas.1413018111. [PMID] 25331888.
2014
Residue specific partitioning of KL4 into phospholipid bilayers
Biochimica et Biophysica Acta (BBA) – Biomembranes. 1838(12):3212-3219 [DOI] 10.1016/j.bbamem.2014.09.006.
2013
Selective Modification of Lipid Bilayer Dynamics and Fluidity Properties By Kl4 (Mimetic of Surfactant Protein B)
Biophysical Journal. 104
2012
A Metastable Liquid Precursor Phase of Calcium Carbonate and Its Interactions With Polyaspartate
Faraday Discussions. 159:291-312 [DOI] 10.1039/c2fd20080e.
2010
Effects of the endosomal lipid bis(monoacylglycero)phosphate on the thermotropic properties of DPPC: A 2H NMR and spin label EPR study.
Chemistry and physics of lipids. 163(7):703-11 [DOI] 10.1016/j.chemphyslip.2010.06.002. [PMID] 20599855.
2010
Lipid polymorphism induced by surfactant peptide SP-B(1-25).
Biophysical journal. 99(6):1773-82 [DOI] 10.1016/j.bpj.2010.06.076. [PMID] 20858421.
2010
Partitioning, dynamics, and orientation of lung surfactant peptide KL(4) in phospholipid bilayers.
Biochimica et biophysica acta. 1798(2):216-22 [DOI] 10.1016/j.bbamem.2009.08.020. [PMID] 19735643.
2009
A low-E magic angle spinning probe for biological solid state NMR at 750 MHz.
Journal of magnetic resonance (San Diego, Calif. : 1997). 197(2):135-44 [DOI] 10.1016/j.jmr.2008.12.008. [PMID] 19138870.
2009
Penetration depth of surfactant peptide KL4 into membranes is determined by fatty acid saturation.
Biophysical journal. 96(10):4085-98 [DOI] 10.1016/j.bpj.2008.12.3966. [PMID] 19450480.
2008
Determination of peptide backbone torsion angles using double-quantum dipolar recoupling solid-state NMR spectroscopy.
Journal of the American Chemical Society. 130(7):2202-12 [DOI] 10.1021/ja074244w. [PMID] 18220389.
2008
Interactions of the C-terminus of lung surfactant protein B with lipid bilayers are modulated by acyl chain saturation.
Biochimica et biophysica acta. 1778(11):2544-54 [DOI] 10.1016/j.bbamem.2008.07.013. [PMID] 18694722.
2008
Phys 548-a Molecular Understanding of Lung Surfactant Derived From Ssnmr Experiments
. 236
2008
Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes.
Colloids and surfaces. B, Biointerfaces. 65(2):178-85 [DOI] 10.1016/j.colsurfb.2008.04.015. [PMID] 18602253.
2008
The helical structure of surfactant peptide KL4 when bound to POPC: POPG lipid vesicles.
Biochemistry. 47(32):8292-300 [DOI] 10.1021/bi702551c. [PMID] 18636713.
2007
A high resolution structure of the putative hinge region in M2 channel-lining segments of the nicotinic acetylcholine receptor.
Biochimica et biophysica acta. 1768(12):2961-70 [PMID] 17996723.
2007
Functional ion channels in tethered bilayer membranes–implications for biosensors.
Chembiochem : a European journal of chemical biology. 8(11):1246-50 [PMID] 17583881.
2007
Incorporation of isotopically enriched amino acids.
Current protocols in protein science. Chapter 26 [DOI] 10.1002/0471140864.ps2603s47. [PMID] 18429325.
2007
Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
Magnetic resonance in chemistry : MRC. 45 Suppl 1:S209-20 [DOI] 10.1002/mrc.2146. [PMID] 18157844.
Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase α-aminoacrylate intermediate
. [DOI] 10.1101/2021.05.12.443852.

Grants

Jul 2021 ACTIVE
National Resource for Advanced NMR Technology
Role: Principal Investigator
Funding: FL STATE UNIV via NATL INST OF HLTH NIGMS
Jul 2021 ACTIVE
Seven tesla preclinical MRI/S scanner for structural, functional and molecular imaging
Role: Principal Investigator
Funding: NATL INST OF HLTH OD
Jul 2020 – Jun 2021
National Resource for Advanced NMR Technology
Role: Principal Investigator
Funding: FL STATE UNIV via NATL INST OF HLTH NIGMS
Jun 2020 – Jun 2022
NMR console upgrade for structural biology and metabolomics
Role: Principal Investigator
Funding: NATL INST OF HLTH OD
Jul 2019 – Jun 2020
National Resource for Advanced NMR Technology
Role: Principal Investigator
Funding: FL STATE UNIV via NATL INST OF HLTH NIGMS
Aug 2018 – Jul 2022
Oxidant and environmental toxicant-induced effects compromise ligation in DNA repair
Role: Other
Funding: NATL INST OF HLTH NIEHS
Jan 2018 ACTIVE
National High Magnetic Field Laboratory Renewal 2018-2022
Role: Principal Investigator
Funding: FL STATE UNIV via NATL SCIENCE FOU
Aug 2017 – Jun 2019
National Resource for Advanced NMR Technology
Role: Principal Investigator
Funding: FL STATE UNIV via NATL INST OF HLTH NIGMS
Apr 2017 – Mar 2018
Advanced 3T for Structural and Functional Imaging at University of Florida
Role: Principal Investigator
Funding: NATL INST OF HLTH OD
Mar 2017 ACTIVE
Functional amyloid formation in streptococcus mutans
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Jan 2013 – Dec 2018
DSR Matching Support – NHMFL Project 2013-2017
Role: Principal Investigator
Funding: UF DIV OF SPONSORED RES MATCHING FUNDS
Jan 2013 – Dec 2018
National High Magnetic Field Laboratory
Role: Principal Investigator
Funding: FL STATE UNIV via NATL SCIENCE FOU
Mar 2012 – Feb 2017
Functional Amyloid Formation in Streptococcus mutans
Role: Project Manager
Funding: NATL INST OF HLTH NIDCR

Contact Details

Phones:
Business:
(352) 294-8399
Emails:
Business:
jrlong@ufl.edu
Addresses:
Business Mailing:
PO Box 100245
GAINESVILLE FL 32610
Business Street:
MBI LG-187
GAINESVILLE FL 326100001