Mingyi Xie

Mingyi Xie,

AST PROF

Department: MD-BIOCHEM / MOLECULAR BIOL
Business Phone: (352) 273-8171
Business Email: mingyi.xie@ufl.edu

About Mingyi Xie

From 2005 to 2010, Dr. Xie’s Ph.D. work focused on investigating the structure and function of vertebrate telomerase, under the guidance of Dr. Julian Chen at Arizona State University. In 2010, he joined the laboratory of Dr. Joan Steitz at Yale University to study non-canonical microRNA (miRNA) biogenesis in Herpesviruses and their mammalian hosts. Dr. Xie joined the faculty at the University of Florida in September, 2016.

Accomplishments

Exemplary Teacher Award
2020 · College of Medicine, University of Florida
Maximize Investigator's Research Award (R35)
2018 · National Institute of General Medical Sciences
Pathway to Independence Award (K99/R00)
2014 · National Cancer Institute
Fellow Award
2012 · Leukemia & Lymphoma Society
Leslie H. Warner Postdoc Fellow
2010 · Yale Medical School
Outstanding Graduate Research Assistant
2009 · Department of Chemistry and Biochemistry, Arizona State University
Award for Advanced Individual in Extra-curricular Scientific and Technological Activity
2003 · School of Life Sciences, Xiamen University

Research Profile

Gene expression involves delicate regulation by RNA-binding proteins (RBP), and enzymes that process and modify RNAs. The overarching goal of our research is to understand how non-coding RNAs and RBPs contribute to gene regulation. At present, my lab focuses on two research directions: microRNA lifecycle microRNAs have been shown to affect diverse cellular pathways critical to human development and disease, underscoring the need to elucidate the mechanism by which their levels are regulated. In this direction, we will investigate microRNA biogenesis, function and degradation. RNA metabolism mediated by the Integrator complex Integrator is a multi-functional, 14-subunit protein complex that has been linked to a variety of disease states, including developmental failure, neurodegenerative disease, and cancer. The underlying disease-causing mechanism is attributable to Integrator’s ubiquitous role in RNA expression pathways, but its RNA-recognition mechanism is poorly understood. We will identify the Integrator subunit(s) that recognize target RNAs and determine the RNAs regulated by the Integrator complex.

Open Researcher and Contributor ID (ORCID)

0000-0001-7955-197X

Areas of Interest
  • Cancer
  • Genetics
  • RNA

Publications

2021
A noncanonical microRNA derived from the snaR-A noncoding RNA targets a metastasis inhibitor.
RNA (New York, N.Y.). 27(6):694-709 [DOI] 10.1261/rna.078694.121. [PMID] 33795480.
2021
Super-enhancer mediated regulation of adult β-globin gene expression: the role of eRNA and Integrator
Nucleic Acids Research. 49(3):1383-1396 [DOI] 10.1093/nar/gkab002. [PMID] 33476375.
2020
Short Hairpin RNAs for Strand-Specific Small Interfering RNA Production.
Frontiers in bioengineering and biotechnology. 8 [DOI] 10.3389/fbioe.2020.00940. [PMID] 32850763.
2020
UTteR control through miRs: fine-tuning ATXN1 levels to prevent ataxia.
Genes & development. 34(17-18):1107-1109 [DOI] 10.1101/gad.343020.120. [PMID] 32873576.
2019
Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions.
Nature communications. 10(1) [DOI] 10.1038/s41467-019-12355-7. [PMID] 31558711.
2019
Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation.
Bio-protocol. 9(8) [DOI] 10.21769/BioProtoc.3219. [PMID] 31428663.
2018
Dicer cleaves 5′-extended microRNA precursors originating from RNA polymerase II transcription start sites
Nucleic Acids Research. 46(11):5737-5752 [DOI] 10.1093/nar/gky306. [PMID] 29746670.
2018
Immunoprecipitation of Tri-methylated Capped RNA
BIO-PROTOCOL. 8(3) [DOI] 10.21769/bioprotoc.2717.
2018
Near-infrared fluorescent northern blot.
RNA (New York, N.Y.). 24(12):1871-1877 [DOI] 10.1261/rna.068213.118. [PMID] 30201850.
2017
An Exportin-1-dependent microRNA biogenesis pathway during human cell quiescence.
Proceedings of the National Academy of Sciences of the United States of America. 114(25):E4961-E4970 [DOI] 10.1073/pnas.1618732114. [PMID] 28584122.
2016
A proximity-dependent assay for specific RNA–protein interactions in intact cells
RNA. 22(11):1785-1792 [DOI] 10.1261/rna.058248.116. [PMID] 27659050.
2015
The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3′ ends
Genes & Development. 29(14):1552-1564 [DOI] 10.1101/gad.266973.115. [PMID] 26220997.
2015
Viral noncoding RNAs: more surprises
Genes & Development. 29(6):567-584 [DOI] 10.1101/gad.259077.115. [PMID] 25792595.
2014
A self-regulating template in human telomerase
Proceedings of the National Academy of Sciences. 111(31):11311-11316 [DOI] 10.1073/pnas.1402531111. [PMID] 24982163.
2014
Versatile microRNA biogenesis in animals and their viruses
RNA Biology. 11(6):673-681 [DOI] 10.4161/rna.28985. [PMID] 24823351.
2013
Mammalian 5′-Capped MicroRNA Precursors that Generate a Single MicroRNA
Cell. 155(7):1568-1580 [DOI] 10.1016/j.cell.2013.11.027. [PMID] 24360278.
2012
Drosha mediates destabilization of Lin28 mRNA targets
Cell Cycle. 11(19):3590-3598 [DOI] 10.4161/cc.21871. [PMID] 22935707.
2012
RNA/DNA hybrid binding affinity determines telomerase template-translocation efficiency
The EMBO Journal. 31(1):150-161 [DOI] 10.1038/emboj.2011.363. [PMID] 21989387.
2011
A Primate Herpesvirus Uses the Integrator Complex to Generate Viral MicroRNAs
Molecular Cell. 43(6):982-992 [DOI] 10.1016/j.molcel.2011.07.025. [PMID] 21925386.
2010
A novel motif in telomerase reverse transcriptase regulates telomere repeat addition rate and processivity
Nucleic Acids Research. 38(6):1982-1996 [DOI] 10.1093/nar/gkp1198. [PMID] 20044353.
2008
Short telomeres are a risk factor for idiopathic pulmonary fibrosis
Proceedings of the National Academy of Sciences. 105(35):13051-13056 [DOI] 10.1073/pnas.0804280105. [PMID] 18753630.
2008
Structure and Function of the Smallest Vertebrate Telomerase RNA from Teleost Fish
Journal of Biological Chemistry. 283(4):2049-2059 [DOI] 10.1074/jbc.m708032200.
2007
Telomerase Mutations in Families with Idiopathic Pulmonary Fibrosis
New England Journal of Medicine. 356(13):1317-1326 [DOI] 10.1056/nejmoa066157.
2007
Ultrasensitive Label-Free Aptamer-Based Electronic Detection
Angewandte Chemie International Edition. 46(47):9054-9056 [DOI] 10.1002/anie.200703242. [PMID] 17957666.
2006
Rolling-Circle Amplification of a DNA Nanojunction
Angewandte Chemie International Edition. 45(45):7537-7539 [DOI] 10.1002/anie.200602113.
. [DOI] 10.53354/pc.gr.151114.

Grants

Sep 2021 ACTIVE
Examine the contribution of miRNA degradation in oxaliplatin-induced colorectal cancer cell death
Role: Principal Investigator
Funding: PARDEE FOU, ELSA U
Jun 2021 ACTIVE
Target RNAs induce microRNA degradation in apoptotic T cell acute lymphoblastic leukemia cells
Role: Principal Investigator
Funding: FL DEPT OF HLTH LIVE LIKE BELLA
Sep 2018 – Aug 2021
OR-DRPD-ROF2018: Processing of transcription start site (TSS-)microRNAs by Dicer proteins
Role: Principal Investigator
Funding: UF RESEARCH
Aug 2018 ACTIVE
RNA metabolism mediated by the Integrator complex
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS
Apr 2018 – Mar 2020
RNA metabolism mediated by the Integrator complex
Role: Principal Investigator
Funding: UF FOUNDATION
Sep 2016 – Feb 2021
Noncanonical microRNA biogenesis and function in a gamma herpesvirus and mammals
Role: Principal Investigator
Funding: NATL INST OF HLTH NCI
Feb 2015 ACTIVE
UF Health Cancer Center Pilot Project Grants funded through the Florida Consortium of National Cancer Institute Centers Program
Role: Project Manager
Funding: UF HEALTH SHANDS HOSPITAL

Teaching Profile

Courses Taught
2017-2018,2020-2021
BCH5413 Mammalian Molecular Biology and Genetics
2020-2021
BCH4905 Biochemistry Senior Research
2018-2021
GMS6001 Fundamentals of Biomedical Sciences I
2019
BCH7410 Advanced Gene Regulation
2017-2019,2021
BCH6415 Advanced Molecular and Cell Biology
2018
GMS7979 Advanced Research
2018
PLP7979 Advanced Research
2018
GMS7980 Research for Doctoral Dissertation

Contact Details

Phones:
Business:
(352) 273-8171
Emails:
Business:
mingyi.xie@ufl.edu